{"title":"高效液相色谱法测定头孢地洛西原料药和片剂含量的建立与验证","authors":"Kirankumar S V, J. Gnana Babu C., S. H G","doi":"10.14419/ijac.v8i2.30963","DOIUrl":null,"url":null,"abstract":"A novel and simple HPTLC method has been developed for the estimation of Cefadroxil (CFD) in bulk and pharmaceutical dosage form on silica gel precoated aluminum 60F254 plates, (10 cm x10 cm) with 0.25 mm thickness. The separation was carried out using ethyl acetate: chloroform (8:2v/v) as mobile phase. The Chromatographic scanning was carried out at 254 nm. The Rf (± SD) values were found to be 0.38 ± 0.05 for CFD. The linearity for analyte was in the range 100-500 ng/band for CFD with correlation coefficients, r2 = 0.9996. The percentage recovery obtained for CFD was in the range of 99.28-100.13% respectively. The proposed method was optimized and validated as per the ICH guidelines17. ","PeriodicalId":13723,"journal":{"name":"International Journal of Advanced Chemistry","volume":"191 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development and validation of HPTLC method for the estimation of cefadroxil in bulk and tablet dosage form\",\"authors\":\"Kirankumar S V, J. Gnana Babu C., S. H G\",\"doi\":\"10.14419/ijac.v8i2.30963\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A novel and simple HPTLC method has been developed for the estimation of Cefadroxil (CFD) in bulk and pharmaceutical dosage form on silica gel precoated aluminum 60F254 plates, (10 cm x10 cm) with 0.25 mm thickness. The separation was carried out using ethyl acetate: chloroform (8:2v/v) as mobile phase. The Chromatographic scanning was carried out at 254 nm. The Rf (± SD) values were found to be 0.38 ± 0.05 for CFD. The linearity for analyte was in the range 100-500 ng/band for CFD with correlation coefficients, r2 = 0.9996. The percentage recovery obtained for CFD was in the range of 99.28-100.13% respectively. The proposed method was optimized and validated as per the ICH guidelines17. \",\"PeriodicalId\":13723,\"journal\":{\"name\":\"International Journal of Advanced Chemistry\",\"volume\":\"191 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-09-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Advanced Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.14419/ijac.v8i2.30963\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Advanced Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14419/ijac.v8i2.30963","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Development and validation of HPTLC method for the estimation of cefadroxil in bulk and tablet dosage form
A novel and simple HPTLC method has been developed for the estimation of Cefadroxil (CFD) in bulk and pharmaceutical dosage form on silica gel precoated aluminum 60F254 plates, (10 cm x10 cm) with 0.25 mm thickness. The separation was carried out using ethyl acetate: chloroform (8:2v/v) as mobile phase. The Chromatographic scanning was carried out at 254 nm. The Rf (± SD) values were found to be 0.38 ± 0.05 for CFD. The linearity for analyte was in the range 100-500 ng/band for CFD with correlation coefficients, r2 = 0.9996. The percentage recovery obtained for CFD was in the range of 99.28-100.13% respectively. The proposed method was optimized and validated as per the ICH guidelines17.
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