分光光度法同时测定片剂中硫酸阿扎那韦和利托那韦的含量

Disha A. Patel, B. Patel, C. Patel
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引用次数: 6

摘要

背景:利托那韦(RTV)和硫酸阿扎那韦(ATV)是蛋白酶抑制剂,RTV主要用作提高其他蛋白酶抑制剂如ATV的生物利用度的助推器。目的:对新剂型即片剂进行质量评价是十分必要的,因此本工作旨在建立一种灵敏、简便、精确的吸光度校正法同时测定片剂剂型中亚硝胺和亚硝胺含量的方法。材料与方法:采用日本Shimadzu紫外-1700双光束分光光度计,光路长度为1 cm,型号为S Shimadzu -1700,采用2.31版UV- probe软件对10 mm匹配的石英细胞进行光谱测量。标准ATV和RTV由Cipla制药有限公司提供。甲醇购自Finar Chemicals Pvt. Ltd.。结果与结论:以甲醇为溶剂,ATV和RTV的λmax分别为279 nm和240 nm。ATV和RTV的浓度范围分别为30 ~ 90 μg/mL和10 ~ 30 μg/mL,符合Beer-Lambert定律。ATV和RTV的回收率分别为100 ~ 100.33%和100 ~ 101.5%。根据国际协调准则会议,对该方法进行了不同参数的验证。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Spectrophotometric method for simultaneous estimation of atazanavir sulfate and ritonavir in tablet dosage form
Background: Ritonavir (RTV) and atazanavir sulfate (ATV) are protease inhibitor and RTV mostly used as a booster for increasing the bioavailability of other protease inhibitors like ATV. Aims: Quality assessment of the new dosage form of RTV and ATV i.e., tablets is very essential and hence this work deals with to develop sensitive, simple and precise method for simultaneous estimation of ATV and RTV in tablet dosage form by absorbance correction method. Materials and Methods: The present work was carried out on Shimadzu Ultraviolate(UV)-1700 double beam spectrophotometer with 1 cm path length supported by S Shimadzu, model-1700(Japan), UV-Probe software, version 2.31 was used for spectral measurements with 10 mm matched quartz cells. Standard ATV and RTV were supplied by Cipla Pharmaceutical Ltd. Methanol was purchased from Finar Chemicals Pvt. Ltd. Results and Conclusion: The λmax or the absorption maxima for ATV and RTV were found to be 279 and 240 nm, respectively in methanol as solvent. The drugs follow Beer-Lambert′s law in the concentration range 30-90 and 10-30 μg/mL for ATV and RTV, respectively. The percentage recovery was found to be 100-100.33% and 100-101.5% for ATV and RTV, respectively. The method was validated for different parameters as per the International Conference for Harmonization Guidelines.
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