植物寄生线虫潜在生物防治剂产酶溶杆菌B25的鉴定及其作用方式

IF 2.7 Q3 MICROBIOLOGY
Sònia Martínez-Servat, Lola Pinyol-Escala, Oriol Daura-Pich, Marta Almazán, Iker Hernández, Belén López-García, Carolina Fernández
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引用次数: 4

摘要

由于植物病原线虫的作用而造成的生产力损失当然很难估计,但它可能占世界农业生产的12%左右。虽然有许多工具可以减少这些线虫的影响,但人们越来越关注它们对环境的影响。酶原溶菌B25是一种有效的植物寄生线虫生物防治剂,对植物根结线虫(rootknot nemate线虫,如Meloidogyne incognita和Meloidogyne javanica)具有防治作用。本文研究了B25对番茄植株RKN侵染的防治效果。杜林塔)被描述。该细菌以平均浓度约108 CFU/mL施用4次,根据种群和病原体压力的不同,效果为50- 95%。此外,B25的防治活性与所使用的对照化学品相当。本文对L. enzymatic genes B25进行了鉴定,并对其作用模式进行了研究,重点研究了其不同的机制,包括运动性、裂解酶和次级代谢产物的产生以及诱导植物防御。不知名m.a cognita的存在增加了B25的抽搐运动性。此外,在贫培养基和富培养基中培养B25后获得的无细胞上清液均能有效抑制RKN卵的体外孵化。这种杀线虫活性对高温敏感,表明其主要是由胞外裂解酶引起的。在培养滤液中鉴定出了次生代谢产物热稳定抗真菌因子和丙胺A/B,并讨论了它们对B25杀线虫活性的贡献。本研究指出L.酶原B25是一种很有前途的植物线虫防治微生物,是开发可持续的杀线虫产品的良好候选。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Characterization of <i>Lysobacter enzymogenes</i> B25, a potential biological control agent of plant-parasitic nematodes, and its mode of action.

Characterization of <i>Lysobacter enzymogenes</i> B25, a potential biological control agent of plant-parasitic nematodes, and its mode of action.

Characterization of <i>Lysobacter enzymogenes</i> B25, a potential biological control agent of plant-parasitic nematodes, and its mode of action.

Characterization of Lysobacter enzymogenes B25, a potential biological control agent of plant-parasitic nematodes, and its mode of action.

It is certainly difficult to estimate productivity losses due to the action of phytopathogenic nematodes but it might be about 12 % of world agricultural production. Although there are numerous tools to reduce the effect of these nematodes, there is growing concern about their environmental impact. Lysobacter enzymogenes B25 is an effective biological control agent against plant-parasitic nematodes, showing control over root-knot nematodes (RKN) such as Meloidogyne incognita and Meloidogyne javanica. In this paper, the efficacy of B25 to control RKN infestation in tomato plants (Solanum lycopersicum cv. Durinta) is described. The bacterium was applied 4 times at an average of concentration around 108 CFU/mL showing an efficacy of 50-95 % depending on the population and the pressure of the pathogen. Furthermore, the control activity of B25 was comparable to that of the reference chemical used. L. enzymogenes B25 is hereby characterized, and its mode of action studied, focusing on different mechanisms that include motility, the production of lytic enzymes and secondary metabolites and the induction of plant defenses. The presence of M. incognita increased the twitching motility of B25. In addition, cell-free supernatants obtained after growing B25, in both poor and rich media, showed efficacy in inhibiting RKN egg hatching in vitro. This nematicidal activity was sensitive to high temperatures, suggesting that it is mainly due to extracellular lytic enzymes. The secondary metabolites heat-stable antifungal factor and alteramide A/B were identified in the culture filtrate and their contribution to the nematicidal activity of B25 is discussed. This study points out L. enzymogenes B25 as a promising biocontrol microorganism against nematode infestation of plants and a good candidate to develop a sustainable nematicidal product.

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来源期刊
AIMS Microbiology
AIMS Microbiology MICROBIOLOGY-
CiteScore
7.00
自引率
2.10%
发文量
22
审稿时长
8 weeks
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