利用分子标记研究地方玫瑰品种的遗传多样性

A. Saidi, Yazdan Eghbalnegad, Z. Hajibarat
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引用次数: 25

摘要

本研究对一个由玫瑰品种组成的种质资源进行了遗传多样性评价。采用起始密码子靶向(SCoT)、保守DNA衍生多态性(CDDP)和直接扩增的微卫星DNA (DAMD)三种不同的分子标记技术估算遗传距离。结果表明,SCoT、CDDP和DAMD标记的平均多态性信息含量分别为0.37、0.36和0.36,表明所研究的标记类型在评估多样性方面是相同的。采用SCoT和CDDP聚类分析将品种划分为4个不同的聚类,而采用DAMD标记数据将品种划分为3个聚类。SCoT所得相似矩阵与CDDP呈显著正相关(r=0.92, p<0.01)。结果表明,SCOT、CDDP和DAMD标记在品种指纹图谱中的效率基本一致。这是首次在一组玫瑰样品中对这三种分子标记的效率进行比较。结果表明,所研究的标记具有适当的多态性,适合用于月季遗传多样性的研究。关键词:指纹,PIC,分子标记,遗传距离,相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study of genetic diversity in local rose varieties (Rosa spp.) using molecular markers
This study was undertaken to evaluate genetic diversity in a germplasm consisting of rose varieties. Genetic distances were estimated using three different molecular marker techniques including: start codon targeted (SCoT), conserved DNA–Derived Polymorphism (CDDP) and directly amplified minisatellite DNA (DAMD). According to the results, the average polymorphism information content was 0.37, 0.36, and 0.36 for SCoT, CDDP and DAMD markers, respectively indicating that the studied marker types were equal in terms of assessing diversity. Cluster analysis using SCoT and CDDP divided the varieties to four distinct clusters whereas DAMD markers data, grouped the varieties into three clusters. There was a positive significant correlation (r=0.92, p<0.01) between similarity matrix obtained by SCoT and CDDP. Results suggested that the efficiency of SCOT, CDDP and DAMD markers had a relatively same efficiency in fingerprinting of varieties. This is the first time that the efficiency of the three molecular markers have been compared with each other in a set of rose samples. The results showed that the studied markers had an appropriate polymorphism and thus were suitable for the study of genetic diversity in rose. Keyword: Fingerprinting, PIC, molecular marker, genetic distance, correlation.
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