Pengaruh Perbedaan Pelarut dan Optimasi Waktu Inkubasi Bakteri Propionibacterium acnes Terhadap Ekstrak Etanol 96% Umbi Bawang Dayak (Eleutherine americana Merr.)

Background: Acne (acne vulgaris) is still a major health problem. Treatment with antibiotics raises problems in the form of resistance, irritation to immunohypersensitivity. Alternatives derived from natural ingredients are needed to avoid these problems, namely Dayak onions.Objective: extracts incubation time of P. acnes bacteria through differences and using an ideal solvent to find out how effective 96% ethanol extract of Dayak onion bulbs inhibits P.acnes.Method: Testing of compounds and the ability of extracts to inhibit bacteria with variations in incubation time, 24hours and 48hours, as well as the use of different solvents, Na-CMC and DMSO, then data analysis was carried-out using SPSSResults: Dayak onion extract contains alkaloids, saponins, flavonoids and triterpenoids. Na-CMC solvent obtained the highest inhibition ability of Dayak onion extract at a concentration of 40% with an inhibition value of 10.12±1.840mm(24hour) and 11.575±1.694 mm(48hour) while DMSO solvent the highest inhibition at a concentration of 20% with inhibition values ​​15.48±1.198 mm(24hours) and 15.91±1.625 mm(48hours). Interpretation of the results of the Wilcoxon test based on ranking, showed a positive difference at 48hours of incubation.Conclusion: The optimal incubation time of P. acnes was 48hours with the best solvent that could be used was dimethyl sulfoxide (DMSO).