通过基因重组强化甘薯子代以提高根干物质和微量养分密度

A. Okereke, U. O. Aguwa, G. Nwaigwe, I. Nwankwo, N. Amanze
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引用次数: 0

摘要

试验在尼日利亚东南部雨林农业生态区Umudike国家块根作物研究所甘薯繁育场雨养条件下进行,产生1万粒植物种子,用于进一步评价高根产量、干物质和微量营养素含量、鉴定高开花亲本和选择亲本进行进一步遗传重组。双亲本杂交的14个亲本采用完全随机区组设计,每区7个亲本,重复3次,其余7个亲本作为花粉供体保持在不同的区组。收集的数据包括:目测出花总数、覆盖花总数、授粉花总数、形成蒴果总数、流产花总数、杂交获得种子总数、好种子数量、未灌浆种子数量、好种子百分比。收集到的数据使用社会科学统计软件包(SPSS, version 15.0)进行统计分析。均值之间的差异采用方差分析(ANOVA)进行检验,均值分离采用差异均值的标准误差在5%的概率下进行。结果表明,尽管对照杂交的花总数看似很高(14520),但其中只有17.4%产出好种子。由于灌种过程中严重的水分胁迫导致了大量的败育现象,未能实现人工授粉10000粒植物种子的目标。品种Tio-joe的花产量最高,为2334。UMUSPO 3与NRSP/2杂交获得了347粒较高的荚果数,表明两亲本亲和性较高,表明两亲本可以进一步进行重组以增强微营养。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fortification of sweet potato progenies for enhanced root dry matter and micro-nutrient density through genetic recombination
Experiment was conducted in the rainforest agro-eco-zone of Southeastern Nigeria under rain fed condition at the sweet potato breeding ground of National Root Crops Research Institute, Umudike, Nigeria to generate 10,000 botanical seeds for further evaluation for high root yield, dry matter and micro-nutrient content, to identify high flowering parent(s) and to select compatible parent(s) for further genetic recombination. The treatment which consisted of 14 parents of bi-parental crossing was laid out in a randomized complete block design with seven parents, each per plot per block and replicated three times while the other seven parents were maintained in different plots as pollen donors. Data collected were on: total number of emerged flowers visually counted, total number of flowers covered, total number of flowers pollinated, total number of capsules formed, total number of flowers aborted, total number of seeds obtained from crosses, number of good seeds, number of unfilled seeds, percentage of good seeds. Data collected were statistically analyzed using Statistical Package for Social Sciences (SPSS, version 15.0). Differences between means were tested using analysis of variance (ANOVA) while means separation was done using standard error of difference means at 5% probability. The results obtained showed that despite the seemingly high total number of flowers produced from the controlled crosses (14520), only 17.4% of this figure yielded good seeds. The objective of realizing 10,000 botanical seeds through the hand pollination was not achieved as a result of high flower abortion arising probably from moisture stress which was severe during seed filling. The variety Tio-joe gave the highest flower production of 2334. The relatively high number of capsules of 347obtained from the cross between UMUSPO 3 and NRSP/2 indicated high compatibility between the two parents which suggested that the two parents could be used for further recombination for micro-nutrient enhancement.
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