结肠癌和直肠癌活检细胞系培养中细菌活性的研究

Q3 Pharmacology, Toxicology and Pharmaceutics

Biomedical and Pharmacology Journal Pub Date : 2023-06-30 DOI:10.13005/10.13005/bpj/2705

Ghofran Khald khlf, S. A. Alash

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引用次数: 0

摘要

结直肠癌(CRC)是全球最常见的疾病和死亡原因。本研究的目的是调查和检测一些干扰结直肠癌发生和进展的细菌。本研究于2022年9月至2023年2月期间进行，共从确诊的CRC患者中采集了50例标本。另外，选取健康志愿者粪便标本50份作为对照组。采用培养培养基和差异培养基(血琼脂、麦康基琼脂和辉瑞琼脂)以及VITEK- 2紧凑系统对收集的所有标本进行细菌的分离和鉴定。对照组标本中细菌种类为大肠埃希菌50种(86.20%)、肺炎克雷伯菌3种(5.17%)、伤寒沙门菌2种(3.44%)、金黄色葡萄球菌1种(1.72%)、奇异变形杆菌1种(1.72%)、铜绿假单胞菌1种(1.72%)，结直肠癌和息肉标本中细菌种类为大肠埃希菌30种(38.69%)、ubercoccus 6种(7.79%)、cloacae肠杆菌4种(5.19%)、奇异变形杆菌11种(14.28)、constellatcoccus phyneis 1种(1.29%)、lutecoccus luteus 1种(1.29%)、假中间葡萄球菌1号(1.29%)、胸拉链球菌1号(1.29%)、弗氏柠檬酸杆菌1号(1.29%)、变形链球菌1号(1.29%)、屎肠球菌5号(6.49%)、粪肠球菌4号(5.19%)、秀丽细粒菌1号(1.29%)、鸡肠球菌2号(2.59%)、粘质沙雷氏菌1号(1.29%)、桑格尼链球菌1号(1.29%)、香型葡萄球菌1号(1.29%)、睾酮常见菌1号(1.29%)、摩根氏菌1号(1.29%)、铜绿假单胞菌1号(1.29%)、肺炎克雷伯菌2号(2.59%)。在结直肠癌组织标本中与结直肠癌相关且数量较多的细菌是埃希氏菌。大肠杆菌30(38.96%)、ubercoccus 6(7.79%)、阴沟肠杆菌4(5.19%)、屎肠球菌5(6.49%)、屎肠球菌4(5.19%)。细胞系培养结果显示，5种细菌的细胞存活率依次为:ubercoccus(16.12%)、Enterococcus faecium(16.39%)、Enterococcus faecalis(9.48%)、Enterobacter cloacae(15.11%)和escherhia coli(17.61%)。使用SPSS对结果进行统计研究，结果显示优秀或(高度)显著(p值在0.001范围内)。

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Study the Bacterial Activity Isolated from Colon and Rectal Cancer Biopsy in Cell Lines Culture

Colorectal cancer (CRC) is the most common disease and cause of death globally. The aim of the study is investigation and detection of some bacterial interfering with CRC occurrence and progression. The study conducted between September 2022 till February 2023, a total of 50 specimens were collected from confirmed CRC patients. In addition, 50 stool specimens were collected from Healthy volunteers, considers as control group. Isolation and identification of bacteria in all collected specimens were done by using cultural and differential media (blood agar, macconkey agar and Pfizer agar), as well as the VITEK- 2 compact system. The bacterial species, in the specimens of control were ( Escherichia coli 50 (86.20%), Klebsiella Pneumonia 3(5.17%), Salmonella typhi 2(3.44%), Staphylococcus aureus 1(1.72%), Proteus mirabilis 1(1.72%) and Pseudomonas aeruginosa 1(1.72%), while in the specimens of CRC and polyp were (Escherichia coli 30(38.69%), Streptococcus uberis 6(7.79%), Enterobacter cloacae 4(5.19%), Proteus mirabilis 11(14.28), Streptococcus constellatus pharyneis 1(1.29%), Micrococcus luteus 1(1.29%), Staphlococcus pseudintermedius 1(1.29%), Streptococcus thoraltensis 1(1.29%), Citrobacter freundii 1(1.29%), Streptoccus mutans 1(1.29%), Enterococcus faecium 5(6.49%), Enterococcus faecalis 4(5.19%), Granulicatella elegans 1(1.29%), Enterococcus gallinarum 2(2.59%), Serratia marcescens 1(1.29%), Streptococcus sangunis 1(1.29%), Staphylococcus lentus 1(1.29%), Comamons testosteroni 1(1.29%), Morganella morganii 1(1.29%), Pseudomonas aeruginosa 1(1.29%), Klebsiella pneumonia 2(2.59%). The bacteria which has been shown to be associated and more abundance in the specimens of CRC tissues are Escherichia.coli 30(38.96%), Streptococcus uberis 6(7.79%), Enterobacter cloacae 4(5.19%), Enterococcus faecium 5(6.49%), Enterococcus faecalis 4(5.19%). Cell-line culture techniques for the five species showed a cellular viability, sequentially Streptocccus uberis (16.12%), Enterococcus faecium (16.39%), Entreococcus faecalis (9.48%), Enterobacter cloacae (15.11%) and Escherichia coli (17.61%). The results statistically studied by using SPSS, which showed excellent or (highly) significant (p-value is in the range of 0.001).

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来源期刊

Biomedical and Pharmacology Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

1.20

自引率

0.00%

发文量

189

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