{"title":"超还原水对三种正畸托槽中变形链球菌的杀菌及生物膜去除效果。","authors":"Hajime Shiiki, Sho Goto, Hiroaki Katsuragi, Sakurako Kobayashi","doi":"10.2334/josnusd.22-0320","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the bactericidal and biofilm removal effect of super reducing water (SRW) on Streptococcus mutans (S. mutans) adhered to orthodontic brackets, in vitro.</p><p><strong>Methods: </strong>Three types of brackets were bonded to aluminum disks. After the formation of S. mutans biofilms on the surfaces, the brackets were divided into three groups (n = 44 each) based on their exposure to SRW: group 1, no treatment; group 2, treated for 5 min; and group 3, treated for 10 min. Total viable counts, adenosine triphosphate measurements, crystal violet assay, and scanning electron microscopy were used to evaluate the effect of SRW.</p><p><strong>Results: </strong>The bacterial counts in groups 2 and 3 were significantly lower than those in group 1 (P < 0.001); however, no significant differences were observed between groups 2 and 3. Marked decreases in the number of bacterial colonies and extent of biofilm formation were observed in groups 2 and 3 compared to group 1. No significant differences in the number of bacterial colonies and amount of biofilm were observed among the three types of brackets in each group.</p><p><strong>Conclusion: </strong>These findings indicate the bactericidal and biofilm removal effect of SRW treatment on S. mutans adhered to orthodontic brackets.</p>","PeriodicalId":16646,"journal":{"name":"Journal of oral science","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The bactericidal and biofilm removal effect of super reducing water on Streptococcus mutans in three types of orthodontic brackets.\",\"authors\":\"Hajime Shiiki, Sho Goto, Hiroaki Katsuragi, Sakurako Kobayashi\",\"doi\":\"10.2334/josnusd.22-0320\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>To investigate the bactericidal and biofilm removal effect of super reducing water (SRW) on Streptococcus mutans (S. mutans) adhered to orthodontic brackets, in vitro.</p><p><strong>Methods: </strong>Three types of brackets were bonded to aluminum disks. After the formation of S. mutans biofilms on the surfaces, the brackets were divided into three groups (n = 44 each) based on their exposure to SRW: group 1, no treatment; group 2, treated for 5 min; and group 3, treated for 10 min. Total viable counts, adenosine triphosphate measurements, crystal violet assay, and scanning electron microscopy were used to evaluate the effect of SRW.</p><p><strong>Results: </strong>The bacterial counts in groups 2 and 3 were significantly lower than those in group 1 (P < 0.001); however, no significant differences were observed between groups 2 and 3. Marked decreases in the number of bacterial colonies and extent of biofilm formation were observed in groups 2 and 3 compared to group 1. No significant differences in the number of bacterial colonies and amount of biofilm were observed among the three types of brackets in each group.</p><p><strong>Conclusion: </strong>These findings indicate the bactericidal and biofilm removal effect of SRW treatment on S. mutans adhered to orthodontic brackets.</p>\",\"PeriodicalId\":16646,\"journal\":{\"name\":\"Journal of oral science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of oral science\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.2334/josnusd.22-0320\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral science","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.2334/josnusd.22-0320","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
The bactericidal and biofilm removal effect of super reducing water on Streptococcus mutans in three types of orthodontic brackets.
Purpose: To investigate the bactericidal and biofilm removal effect of super reducing water (SRW) on Streptococcus mutans (S. mutans) adhered to orthodontic brackets, in vitro.
Methods: Three types of brackets were bonded to aluminum disks. After the formation of S. mutans biofilms on the surfaces, the brackets were divided into three groups (n = 44 each) based on their exposure to SRW: group 1, no treatment; group 2, treated for 5 min; and group 3, treated for 10 min. Total viable counts, adenosine triphosphate measurements, crystal violet assay, and scanning electron microscopy were used to evaluate the effect of SRW.
Results: The bacterial counts in groups 2 and 3 were significantly lower than those in group 1 (P < 0.001); however, no significant differences were observed between groups 2 and 3. Marked decreases in the number of bacterial colonies and extent of biofilm formation were observed in groups 2 and 3 compared to group 1. No significant differences in the number of bacterial colonies and amount of biofilm were observed among the three types of brackets in each group.
Conclusion: These findings indicate the bactericidal and biofilm removal effect of SRW treatment on S. mutans adhered to orthodontic brackets.
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