操作35s标记分子的安全工作站的优化:体外细胞代谢标记的一个案例

IF 1.4 4区医学 Q4 ENVIRONMENTAL SCIENCES

Radioprotection Pub Date : 2023-04-25 DOI:10.1051/radiopro/2023015

M. Audry, M. Coureuil, A. Hinzpeter, P. Frot, O. Saltiel, M. Kadri, M-L Gaab, A-S Armand, N. Servel

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引用次数: 0

摘要

涉及35S标记分子的脉冲追踪或细胞代谢实验被认为是挥发性的。供应商和文献都没有提出任何限制污染的解决方案。本研究的目的就是填补这一空白。在这里，我们暴露了通常的程序，包括使用可用的设备。由于该程序会导致操作人员的内部和外部污染，我们测试了一个更安全的工作站，配备了集体保护设备，我们验证了该设备可以限制工人的污染。本研究提出了一种以更安全的方式使用35S标记分子的方法，可应用于任何实验室。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Optimization of Secure Workstation for manipulation of 35S-labelled molecules: a case of in vitro cell metabolic labelling

Pulse-chase or cell metabolic experiments involving 35S labelled molecules are deemed to be volatile. Neither the suppliers nor the literature propose any solutions to limit contamination. The aim of this study is to fill in this gap. Here, we expose the usual procedures, which consist in working with the available equipment. As this procedure leads to internal and external contaminations of the operators, we tested a more secure workstation with collective protection equipment that we validated as limiting contaminations for workers. This study proposes a method to use 35S labelled molecules in a more secure way, which could be applied in any laboratory.

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来源期刊

Radioprotection ENVIRONMENTAL SCIENCES-PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

CiteScore

3.30

自引率

54.50%

发文量

审稿时长

>12 weeks

期刊介绍： Radioprotection publishes articles on all aspects of radiological protection, including non-ionising as well as ionising radiations. Fields of interest range from research, development and theory to operational matters, education and training. The very wide spectrum of its topics includes (theoretical and practical aspects): dosimetry, instrument development, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.