Sameeran Gam, Suman Kumar, Susankar Kushari, Rajat Subhra Dutta, Himangshu Sarma, Arpita Paul, Md Kamaruz Zaman
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引用次数: 0
摘要
Oroxylum indicum 是一种著名的传统药用植物，在亚洲被用来缓解各种疾病。该研究旨在利用 HPTLC 技术鉴定 O. indicum 茎皮中的生物活性化合物。此外，还测定了植物提取物对 HeLa（人宫颈癌）细胞系的细胞毒性作用。研究结果表明，植物中存在黄酮类、酚类、单宁酸和类固醇等植物成分。MTT（3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四氮唑）测定显示，O. indicum 的乙醇、甲醇和水提取物对 HeLa 细胞株具有细胞毒性作用，IC50 值分别为 119、89.43 和 114.1 µg/mL，而标准多柔比星的 IC50 值为 3.895 µg/mL。目前的研究表明，O. indicum 的甲醇提取物可能具有化学预防特性。不过，还需要进行更多的研究，以分离和鉴定 O. indicum 中存在的特定化学实体。这些研究将有助于确定有望成为天然抗癌剂的潜在先导化合物。
Phytochemical Screening, HPTLC Fingerprinting and Evaluation of In Vitro Cytotoxic Activity of Stem Bark Extracts of Oroxylum indicum (L.) Vent. Against Human Cervical Cancer Cells.
Oroxylum indicum, a well-known traditional medicinal plant which is used to alleviate various kinds of diseases in Asia. The study aimed to identify bioactive compounds present in O. indicum stem bark using HPTLC technique. Further, the cytotoxic effects of the plant extracts were determined against HeLa (human cervical carcinoma) cell lines. The results of the study have shown the presence of the phytoconstituents such as flavonoids, phenols, tannins and steroids. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) assay showed that the ethanol, methanol and water extracts of O. indicum exhibited cytotoxic effect in HeLa cell lines with IC50 values of 119, 89.43 and 114.1 µg/mL, respectively against standard doxorubicin with IC50 value 3.895 µg/mL. The current study suggests that the methanol extract of O. indicum may offer chemopreventive properties. However, additional research is required to isolate and characterize the specific chemical entities present in O. indicum. These studies will aid in identifying a potential lead compound that holds promise as a natural anticancer agent.
期刊介绍：
The primary mission of the journal is to promote improvement in the health and well-being of community through the development and practice of clinical biochemistry and dissemination of knowledge and recent advances in this discipline among professionals, diagnostics industry, government and non-government organizations. Indian Journal of Clinical Biochemistry (IJCB) publishes peer reviewed articles that contribute to the existing knowledge in all fields of Clinical biochemistry, either experimental or theoretical, particularly deal with the applications of biochemistry, molecular biology, genetics, biotechnology, and immunology to the diagnosis, treatment, monitoring and prevention of human diseases. The articles published also include those covering the analytical and molecular diagnostic techniques, instrumentation, data processing, quality assurance and accreditation aspects of the clinical investigations in which chemistry has played a major role, or laboratory animal studies with biochemical and clinical relevance.
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