三种不同溶剂提取物的植物化学成分筛选及抗氧化活性研究

N. A. Maskam, N. Ravi, H. M. Hassan, M. M. Nor
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引用次数: 1

摘要

本研究对红迭叶三种不同溶剂提取物的化学成分筛选及抗氧化活性进行了研究。样本采集于马来西亚彭亨。采用己烷、二氯甲烷(DCM)和甲醇提取物进行研究。采用建立的常规方法定量测定其总酚、总黄酮含量和抗氧化活性。植物化学筛选试验测定了黄酮类化合物、单宁、皂苷、萜类化合物和酚类化合物的含量。DPPH法测定总抗氧化活性,Follin-Ciocalteau法测定总酚含量(TPC)。筛选结果表明,红毛霉含有所有植物化学成分,但不含皂苷。正己烷、DCM和甲醇植物提取物之间存在显著差异;己烷和DCM提取物的抗氧化活性较强,而甲醇提取物的抗氧化活性较强。由于红椒具有较高的抗氧化性和TPC;它可以作为天然抗氧化剂使用。需要进一步的研究来确定这种高抗氧化活性的确切活性化合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochemicals Screening and Antioxidant Activity of Three Different Solvent Extracts of Euodia Redleyi Leaves
This study was conducted to identify the phytochemicals screening and antioxidant activity of three different solvent extracts of Euodia Redleyi leaves. The sample was collected at Pahang, Malaysia. Hexane, dicholoromethane (DCM) and methanol extracts of the leaves were used in this study. The established conventional methods were used for quantify the total phenol, flavonoids contents and antioxidant activity. Phytochemical screening test determined the presence of flavonoids, tannin, saponin, terpenoid and phenolic content. Total antioxidant activities were determined by DPPH method and Total phenolic content (TPC) were determined by Follin-Ciocalteau method. A screening test for E.redleyi showed the presence of all phytochemical but absence of saponin. There was a significant difference between hexane, DCM and methanol plant extracts; hexane and DCM extracts were more prominent in antioxidant activity but methanol extraction was more prominent in TPC. Since E. redleyi exhibited high antioxidant and TPC; it can be used as a natural antioxidant. Further study needed to identify the exact active compound underlying this high antioxidant activity.
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