马钱子抗原特异性免疫应答的免疫学探索研究

A. Gupta
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引用次数: 0

摘要

用含表面抗原(HBsAg)的乙型肝炎疫苗测定马钱子叶水提物的细胞毒性和抗原特异性免疫活性。20µg/ml)与抗体产生有关,并在裂解的人全血中仔细检查其与细胞因子的增殖反应。在这些研究中,植物化学(定性)分析被确定,并通过高效薄层色谱(HPTLC)和生物无机指纹图谱评估次生代谢物的存在。此外,采用可变剂量(1 ~ 30 mg/ml)马钱子(Strychnos nux-vomica),以HBsAg为包被抗原进行间接Elisa。在这些免疫学研究的继续,抗原特异性免疫反应和细胞毒性通过MTT测定感染的人全血使用HBsAg(20µg/ml, 50µl)。结果表明,马钱子的次生代谢产物和生物无机化合物的含量均在定性和定量的范围内。此外,马钱子抗HBsAg IgG滴度较标准组和对照组有所增强,但与对照组相比,高剂量马钱子抗HBsAg IgG滴度突然下降,细胞因子(IL-2和IL-12)水平也有所下降。我们的数据表明马钱子可能有助于提高HBsAg抗体,但在感染的裂解的人全血中,HBsAg增殖反应和细胞因子(IL-2和IL-12)突然下降,并且在高剂量下也表现出一定的细胞毒性作用。换句话说,马钱子可能是一种有效的B细胞免疫增强剂和T细胞对HBsAg的抑制剂。DOI: http://dx.doi.org/10.5281/zenodo.3244227
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunological exploration based studies on Strychnos nux-vomica regarding antigen specific immune response
In an effort to determine its cytotoxicity as well as antigen specific immune activity of aqueous leaves extract of Strychnos nux-vomica using hepatitis B vaccine containing surface antigen (HBsAg; 20 µg/ml) pertaining to antibody production and scrutinize its proliferative response along with cytokines in lysed human whole blood. For these studies, phytochemical (qualitative) analysis was determined and evaluates the presence of secondary metabolites through high performance thin layer chromatography (HPTLC) and bio-inorganic fingerprinting. In addition, indirect Elisa was performed using HBsAg as coating antigen using variable doses (1-30 mg/ml) of Strychnos nux-vomica. In continuation of these immunological studies, antigen specific immune response along with cytotoxicity was determined through MTT assay in infected human whole blood using HBsAg (20 µg/ml, 50 µl). The results showed that Strychnos nux-vomica showed qualitatively as well as quantitatively determined the presence of secondary metabolites along with bio-inorganic compounds. In addition, Strychnos nux-vomica showed enhancement in anti-HBsAg IgG titre as compared to standard and control but there is sudden decline in proliferation with HBsAg and also showed decline in cytokines (IL-2 and IL-12) level at higher doses as compared to control. Our data suggest that Strychnos nux-vomica may help to raise antibodies against HBsAg but sudden decline in HBsAg proliferative response along with cytokines (IL-2 and IL-12) in infected lysed human whole blood and also showed some cytotoxic effect at higher doses. In other words, Strychnos nux-vomica could be a potent immune enhancer of B cells and inhibitor of T cells against HBsAg. DOI: http://dx.doi.org/10.5281/zenodo.3244227
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