一种产高g-亚麻烯酸的产油真菌的鉴定与特性研究

A. Murad, N. Karim, N. Hashim, A. M. Adnan, Z. Zainal, A. Hamid, F. Bakar
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引用次数: 7

摘要

产油真菌是生产必需多不饱和脂肪酸(PUFA)的良好替代品,如亚麻酸(GLA;十八3)。在这项研究中,我们对一种局部分离的真菌,分离物2A1,进行了脂质和GLA产量的评估,并确定了它的身份。在限氮培养基中培养真菌,每12小时测定脂质和GLA的产量。每细胞干重产脂率最高为38.64% (w/w), GLA产量最高为0.65 g/L。这些观察结果表明,分离物2A1是一个很好的脂质生产者,能够积累大量的GLA。为了在种水平上鉴定分离物2A1,在光镜和电镜下观察其形态。随后,对其18S rDNA和its序列进行克隆、测序,并与相关真菌的18S rDNA和ITS1序列进行系统发育分析。显微镜下观察发现,分离物2A1产生囊胞菌丝和自生球形孢子囊,直径在6 ~ 8 μm之间。基于1.6 kb 18S rDNA区域建立的邻居连接树将分离物2A1与Cunninghamella属真菌聚类。基于154 bp ITS1序列的最大简约树分析将分离物2A1与100% bootstrap值的贝氏Cunninghamella bainieri菌株NRRL 1375组合在一起。因此,基于形态学和分子系统发育数据,分离物2A1被指定为贝氏胞杆菌菌株2A1。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification and Characterisation of an Oleaginous Fungus Producing High g-Linoleneic Acid
Oleaginous fungi serve as good alternatives for the production of essential, polyunsaturated fatty acids (PUFA) such as glinolenic acid (GLA; 18:3). In this study, a locally isolated fungus, isolate 2A1, was evaluated for its lipid and GLA production, and its identity was determined. The fungus was grown in nitrogen-limited media and the amount of lipid and GLA produced were determined every 12 hours. The highest percentage of lipid produced per cell dry weight was 38.64% (w/w) while the highest amount of GLA produced was 0.65 g/L. These observations indicate that isolate 2A1 is a good lipid producer and capable of accumulating high amounts of GLA. To identify isolate 2A1 to the species level, its morphology was observed under the light and electron microscope. Subsequently, its 18S rDNA and the internal transcribed spacer (ITS) sequences were cloned, sequenced and analysed phylogenetically to 18S rDNA and ITS1 sequences of related fungi. Microscopic observation showed that isolate 2A1 produced coenocytic hyphae and self-bearing globose sporangioles, with a diameter between 6-8 μm. Neighbour-joining tree built based on the 1.6 kb 18S rDNA region clustered isolate 2A1 with fungi of the genus Cunninghamella. Maximum parsimony tree analysis based on a 154 bp ITS1 sequence grouped isolate 2A1 together with Cunninghamella bainieri strain NRRL 1375 with 100% bootstrap value. Thus, based on morphological and molecular phylogenetic data, isolate 2A1 is designated as C. baineri strain 2A1.
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