牛血清白蛋白糖探针的研制与应用

Journal of Chemical Research-s Pub Date : 2023-05-01 DOI:10.1177/17475198231177954

X. Gu, Fei Ji, Dawei Jiang, Wenhui Liang, Yan Feng, Shanshan Niu, Qiang Zhao

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引用次数: 0

摘要

设计并合成了由3-乙酰基-5- c -(2,3-二羟基-1,4-无羟基-β- d -红-四呋喃基)-2-甲基呋喃与苯甲醛和1-萘醛缩合而成的两种糖探针GFP和GFN。在这两种探针中，GFP在广泛的pH范围内对磷酸盐缓冲盐水中的牛血清白蛋白显示出选择性和敏感的开关荧光响应。该探针的荧光与牛血清白蛋白浓度在0 ~ 0.8 mg/mL范围内呈良好的线性关系，检测限低至3.7 nM。牛血清白蛋白/绿色荧光蛋白的化学计量比为1:1。本研究为开发用于牛血清白蛋白检测的糖探针铺平了道路。

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The development and application of glyco-probes for bovine serum albumin

Two glyco-probes, GFP and GFN, which are derived from the condensation of 3-acetyl-5-C-(2,3-dihydroxy-1,4-anhydro-β-D-erythro-tetrofuranosyl)-2-methylfuran with benzaldehyde and 1-naphthaldehyde, respectively, are designed and synthesized. Of these two probes, GFP displays a selective and sensitive switch-on fluorescence response towards bovine serum albumin in phosphate-buffered saline over a wide pH range. Moreover, the fluorescence of this probe displays a good linear relationship with the bovine serum albumin concentration ranging from 0 to 0.8 mg/mL, and has a limit of detection as low as 3.7 nM. The stoichiometric ratio of bovine serum albumin /GFP is 1:1. This research paves the way for the development of glyco-probes for bovine serum albumin sensing.

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来源期刊

Journal of Chemical Research-s 化学科学, 有机化学, 有机合成

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Chemical Research is a peer reviewed journal that publishes full-length review and research papers in all branches of experimental chemistry. The journal fills a niche by also publishing short papers, a format which favours particular types of work, e.g. the scope of new reagents or methodology, and the elucidation of the structure of novel compounds. Though welcome, short papers should not result in fragmentation of publication, they should describe a completed piece of work. The Journal is not intended as a vehicle for preliminary publications. The work must meet all the normal criteria for acceptance as regards scientific standards. Papers that contain extensive biological results or material relating to other areas of science may be diverted to more appropriate specialist journals. Areas of coverage include: Organic Chemistry; Inorganic Chemistry; Materials Chemistry; Crystallography; Computational Chemistry.