{"title":"快速转化选择的直接克隆方法","authors":"A. Gomaa, S. Yang, Sangmi Sun, G. Chung","doi":"10.3923/JM.2015.551.556","DOIUrl":null,"url":null,"abstract":"This study highlights a reporter-less direct-cloning protocol based on PCR and digestion/ligation reactions. Few manipulations were done allowing the user to reduce the time required for screening the right colony of any transformed cells. The obtained result showed high efficiency compared to the other commercially-available high throughput techniques, especially Gateway.","PeriodicalId":20888,"journal":{"name":"Research Journal of Microbiology","volume":"12 1","pages":"551-556"},"PeriodicalIF":0.0000,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Direct Cloning Protocol for Rapid Transformant Selection\",\"authors\":\"A. Gomaa, S. Yang, Sangmi Sun, G. Chung\",\"doi\":\"10.3923/JM.2015.551.556\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study highlights a reporter-less direct-cloning protocol based on PCR and digestion/ligation reactions. Few manipulations were done allowing the user to reduce the time required for screening the right colony of any transformed cells. The obtained result showed high efficiency compared to the other commercially-available high throughput techniques, especially Gateway.\",\"PeriodicalId\":20888,\"journal\":{\"name\":\"Research Journal of Microbiology\",\"volume\":\"12 1\",\"pages\":\"551-556\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Research Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3923/JM.2015.551.556\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3923/JM.2015.551.556","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Direct Cloning Protocol for Rapid Transformant Selection
This study highlights a reporter-less direct-cloning protocol based on PCR and digestion/ligation reactions. Few manipulations were done allowing the user to reduce the time required for screening the right colony of any transformed cells. The obtained result showed high efficiency compared to the other commercially-available high throughput techniques, especially Gateway.
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