Effect of Different Chemical Formulation on Agarwood (Aquilaria malaccensis) Resin Weight

Agarwood is described as fragrance, smelling wood that is usually derived from the trunk of genus Aquilaria The objective of this study was to develop and evaluate four chemical-based inoculants to induce karas tree to form agarwood resin. Four chemical inoculants were applied into 6 years old karas tree (Aquilaria malaccensis) plantations in Slim River, Perak, Malaysia using dripping techniques. The inoculant used were labelled as A, B, C and D. Commercial inoculant was also used for comparison purposes and labelled as K while a healthy tree, act as control was labelled as N. After four months of inoculation, these samples were cut down and discoloured tissue samples were collected. Solvent extraction method using dichloromethane (DCM) was carried out to obtain the resin crude extract from plant tissues of the samples. All chemical formulation used were succesfully stimulated sample trees to produce resin. Inoculant B produced the highest crude extract recovery (5.49%) compared to inoculant C (4.78%) followed by A (4.32%), D (2.98%), K (1.07%) and N (0.13%). From this study, inoculant B showed the highest yield of resin. Hence, result from current study has the potential to contribute in enhancing new types of inoculant production for the agarwood industries in Malaysia.