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Q2 Biochemistry, Genetics and Molecular Biology

Current Protocols in Molecular Biology Pub Date : 2019-12-19 DOI:10.1002/cpmb.94

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引用次数: 0

摘要

封面:与Laughery和Wyrick相关(https://doi.org/10.1002/cpmb.110)，封面图片显示了酵母中简单CRISPR基因组编辑的实验策略。中央图像描述了cas9诱导的DNA断裂如何通过与修复模板的同源重组(HR)在酵母中产生无标记基因组编辑。外圈描绘了用于酵母中简单CRISPR/Cas9基因组编辑的Cas9/sgRNA表达载体的组织结构。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Issue Information TOC

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Issue Information TOC

Cover: Related to Laughery and Wyrick (https://doi.org/10.1002/cpmb.110), The cover image shows the experimental strategy for simple CRISPR genome editing in yeast. The central image depicts how Cas9-induced DNA breaks can be used to generate marker-free genome edits in yeast by homologous recombination (HR) with a repair template. The outer circle depicts the organization of a Cas9/sgRNA expression vector used for simple CRISPR/Cas9 genome editing in yeast.

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来源期刊

Current Protocols in Molecular Biology Biochemistry, Genetics and Molecular Biology-Molecular Biology

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