胶体金标记技术增强表面等离子体共振生物传感器的灵敏度

Supramolecular Science Pub Date : 1998-10-01 DOI:10.1016/S0968-5677(98)00105-9

J.H. Gu, H. Lü, Y.W. Chen, L.Y. Liu, P. Wang, J.M. Ma, Z.H. Lu

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引用次数: 24

摘要

在我们的实验中，胶体金被用来增强从表面等离子体共振生物传感器获得的信号。将人IgG分子的Fab '片段固定在金表面，并与溶液中的羊抗人IgG(SAH-IgG)分子反应。结果表明，引入胶体金标记技术后，表面等离子体共振仪的灵敏度提高了约300倍。

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Enhancement of the sensitivity of surface plasmon resonance biosensor with colloidal gold labeling technique

In our experiment colloidal gold was used to enhance the signal obtained from the surface-plasmonresonance biosensor. Fab′ fragments of human IgG molecules were immobilized on the gold surface and reacted with the sheep-anti-human IgG(SAH-IgG) molecules in solution. It was found that when the colloidal gold labeling technique was introduced the sensitivity of surface plasmon resonance instruments increased by a factor of about 300.

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Supramolecular Science

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