Jumpei Hayashi, M. Kawashita, T. Miyazaki, M. Kamitakahara, K. Ioku, H. Kanetaka
{"title":"牛血清白蛋白和骨桥蛋白在羟基磷灰石和氧化铝上吸附行为的比较","authors":"Jumpei Hayashi, M. Kawashita, T. Miyazaki, M. Kamitakahara, K. Ioku, H. Kanetaka","doi":"10.3363/PRB.26.23","DOIUrl":null,"url":null,"abstract":"Adsorption behavior of bovine serum albumin (BSA) and osteopontin (OPN) on osteoconductive hydroxyapatite (HA) and non-osteoconductive alpha-type alumina (-Al2O3) was studied as function of protein concentration and pH, using Bradford dye binding assay. BSA showed a much larger binding capacity on -Al2O3 than that on HA irrespective of BSA solution concentration and pH. Furthermore, OPN is likely to show the same adsorption characteristic as BSA. Definite correlation was not observed between the albumin or OPN adsorption capacity and the osteoconductivity of materials, suggesting that other factors (e.g., orientation, arrangement, etc. of albumin and/or OPN) likely govern expression of the osteoconductivity. (Received February 2, 2012; Accepted March 30, 2012)","PeriodicalId":20022,"journal":{"name":"Phosphorus Research Bulletin","volume":"7 1","pages":"23-28"},"PeriodicalIF":0.0000,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"8","resultStr":"{\"title\":\"COMPARISON OF ADSORPTION BEHAVIOR OF BOVINE SERUM ALBUMIN AND OSTEOPONTIN ON HYDROXYAPATITE AND ALUMINA\",\"authors\":\"Jumpei Hayashi, M. Kawashita, T. Miyazaki, M. Kamitakahara, K. Ioku, H. Kanetaka\",\"doi\":\"10.3363/PRB.26.23\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Adsorption behavior of bovine serum albumin (BSA) and osteopontin (OPN) on osteoconductive hydroxyapatite (HA) and non-osteoconductive alpha-type alumina (-Al2O3) was studied as function of protein concentration and pH, using Bradford dye binding assay. BSA showed a much larger binding capacity on -Al2O3 than that on HA irrespective of BSA solution concentration and pH. Furthermore, OPN is likely to show the same adsorption characteristic as BSA. Definite correlation was not observed between the albumin or OPN adsorption capacity and the osteoconductivity of materials, suggesting that other factors (e.g., orientation, arrangement, etc. of albumin and/or OPN) likely govern expression of the osteoconductivity. (Received February 2, 2012; Accepted March 30, 2012)\",\"PeriodicalId\":20022,\"journal\":{\"name\":\"Phosphorus Research Bulletin\",\"volume\":\"7 1\",\"pages\":\"23-28\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phosphorus Research Bulletin\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3363/PRB.26.23\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phosphorus Research Bulletin","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3363/PRB.26.23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
COMPARISON OF ADSORPTION BEHAVIOR OF BOVINE SERUM ALBUMIN AND OSTEOPONTIN ON HYDROXYAPATITE AND ALUMINA
Adsorption behavior of bovine serum albumin (BSA) and osteopontin (OPN) on osteoconductive hydroxyapatite (HA) and non-osteoconductive alpha-type alumina (-Al2O3) was studied as function of protein concentration and pH, using Bradford dye binding assay. BSA showed a much larger binding capacity on -Al2O3 than that on HA irrespective of BSA solution concentration and pH. Furthermore, OPN is likely to show the same adsorption characteristic as BSA. Definite correlation was not observed between the albumin or OPN adsorption capacity and the osteoconductivity of materials, suggesting that other factors (e.g., orientation, arrangement, etc. of albumin and/or OPN) likely govern expression of the osteoconductivity. (Received February 2, 2012; Accepted March 30, 2012)
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