Effects of essential oils and their combinations added to broiler diets on the mineral contents of some tissues and bone breaking strength

At the end of the experiment, approximately 5 cc of blood was taken from broilers, and the serum was separated by centrifugation at 3000 rpm for 5 min. Serum mineral concentrations were determined directly in an atomic emission spectrometer (AX-ICP, Varian Vista). Subsequently, the tibia was removed from the slaughtered broilers, and the meat and bone marrow were cleaned and dried at 105°C for 24 h. Samples were taken from similar parts of each tibia (middle axis). The mineral content of the tibias was determined with an atomic emission spectrometer (ICP, VISTA AX CCD Simultaneous ICP-AES) after wet decomposition of the tibia in a microwave (Mars Xpress Technology Inside).