大麦幼苗提取物的双歧性研究

Q Engineering
Y. Zakharova, T. Kotova, O. Soboleva, A. Sukhikh, V. Le
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引用次数: 1

摘要

研究对象是大麦种子提取物对双歧杆菌繁殖动力学的影响。一名男子进行了这项研究,以研究不同制剂变体播种的大麦幼苗微芽提取物性质对双歧杆菌属代表菌的体外影响。采用两歧双歧杆菌791和两歧双歧杆菌20两种菌株。干燥的种子在播种前分别暴露在功率为0.42 kW和0.70 kW的微波辐射下,磁控管频率为2.45 GHz,暴露时间为11秒,然后由一名男子连续7天发芽。研究人员用岛津LC-20显像色谱仪测定了提取物中的糖成分。用SF-2000分光光度计(俄罗斯“光谱”实验设计局)监测细菌生长动力学,每小时记录培养皿内容物的光密度,持续20小时。在0.42 kW微波波束处理下,大麦苗提取物对两歧双歧杆菌791和二歧双歧杆菌20在指数期的繁殖有刺激作用。两歧双歧杆菌791在固定阶段的光密度指标较为稳定,主要表现在添加处理能力最大的大麦提取物时。根据色谱数据,三萜类醇含量高,抗氧化剂强;而半乳糖,一种双歧益生元,在这样的制备选择中会增加种子提取物的含量。利用微波处理的大麦提取物进行生物工艺处理,可以缩短双歧杆菌周期性培养的适应时间,在固定生长阶段形成较高的种群密度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bifidogenic Properties of Barley Seedling Extracts
The study subject is the bifidobacteria reproduction kinetics in the presence of barley seed extracts. A man run the research in order to study the influence of the extract nature of barley seedlings microgreens with different preparation variants for sowing on representatives of the genus Bifidobacterium in vitro. There were two strains used: Bifidobacterium bifidum 791 and Bifidobacterium bifidum 20. Dry seeds were exposed to microwave radiation with a power of 0.42 kW and 0.70 kW before sowing, the magnetron frequency was 2.45 GHz, the exposure was 11 sec. Then a man sprouted the grains for seven days. The researchers determined the sugar composition in the extracts by the Shimadzu LC-20 Prominence chromatograph. monitored the bacterial growth kinetics by the spectrophotometer SF-2000 (Experimental Design Bureau “Spectrum”, Russia), recording the optical density of the cuvette contents hourly for 20 hours. There was the reproduction stimulation of B. bifidum 791 and B. bifidum 20 in the exponential phase with an extract from barley seedlings treated with 0.42 kW microwave beams. Moreover, there were stable optical density indicators of B. bifidum 791 in the stationary phase, mainly when adding barley extract with maximum processing power. According to the chromatography data, the trietol content, a powerful antioxidant; and galactose, a bifidogenic prebiotic, increases in seed extracts with such preparation options. A man can use the barley extracts treated with microwave rays in biotechnological processes to reduce the adaptation time of bifidobacteria in periodic culture and to form a high population density in the stationary growth phase.
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来源期刊
Agro Food Industry Hi-tech
Agro Food Industry Hi-tech 工程技术-生物工程与应用微生物
CiteScore
0.29
自引率
0.00%
发文量
0
审稿时长
6-12 weeks
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