Ivana Maldonado , Silvia Relloso , Liliana Guelfand , Bárbara Fox , Natalia Azula , Vanesa Romano , Agostina Cantore , Rubén Barrios , Susana Carnovale , Ezequiel Nuske , Patricia Minervini , members of the Red de Micología de la Ciudad Autónoma de Buenos Aires [Mycology Network of the Autonomous City of Buenos Aires] (RMCABA)
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引用次数: 0
摘要
背景:皮癣菌的鉴定通常是通过形态学分析来完成的。然而,由于新物种和复合体的发现,以及某些分离株由于没有结果,可能会阻碍这一过程。基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)似乎是提高鉴定的一种选择。目的:利用MALDI-TOF MS建立一个用于皮肤真菌鉴定的数据库(DB),包括来自布宜诺斯艾利斯自治城市真菌学网络Red de Micología de la Ciudad Autónoma de Buenos Aires (RMCABA)的32株分离物和1株参考分离物(RMCABA DB),并评估其添加到供应商Bruker (Bruker DB)提供的数据库中的性能。方法:对RMCABA DB中所有分离株进行形态学和测序鉴定。为了评估扩展DB (Bruker DB + RMCABA DB)的性能,纳入了136株临床分离株。结果:种水平鉴定率由45%上升到88%,属水平鉴定率由23%下降到7%;结论:加入包含本地分离株的RMCABA DB后,MALDI-TOF MS在鉴定皮肤真菌方面具有更好的性能。
Evaluation of the MALDI-TOF mass spectrometry technique for the identification of dermatophytes: Use of an extended database
Background
Identification of dermatophytes is usually performed through morphological analyses. However, it may be hindered due to the discovery of new species and complexes and, with some isolates, by the absence of fructification. Matrix-assisted laser desorption/ionisation-time-of-flight mass spectrometry (MALDI-TOF MS) seems to be an option for improving identification.
Aims
To develop a database (DB) for the identification of dermatophytes with MALDI-TOF MS, including 32 isolates from the Red de Micología de la Ciudad Autónoma de Buenos Aires [Mycology Network of the Autonomous City of Buenos Aires] (RMCABA) and one reference isolate (RMCABA DB), and evaluate its performance when added to the DB from the supplier, Bruker (Bruker DB).
Methods
All the isolates in the RMCABA DB were identified based on morphology and sequencing. To evaluate the performance of the extended DB (Bruker DB plus RMCABA DB), 136 clinical isolates were included.
Results
The percentages of identification at the species level increased from 45% to 88%, but the identification at the genus level decreased from 23% to 7%.
Conclusions
MALDI-TOF MS yielded better performance in the identification of dermatophytes after including the RMCABA DB, which encompassed local isolates.
期刊介绍:
Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.