Zhongdang Xiao, Lianyong Su, Jianhua Gu, Zuhong Lu, Yu Wei
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Immunoliposomes assemblied layer by layer from reverse micelles
A method is described for the coupling of the HBsAb antibody on to a liposome by reverse micelles passing through the modified organic/water interface. Incorporation of the fatty acid modified HBsAb into liposomal membranes was studied as a function of the extent of fatty acid coupling. Results show that the incorporation of HBsAb is proportional to the lipid coupling degree. The HBsAb incorporated into the liposomal membrane by this method remains an antigen binding activity shown by the liposome dialysis assay.
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