In situ – Phaseolotoxinproduktion durch permeabilisierte und immobilisierte Zellen von Pseudomonas syringae pv. phaseolicola

Pseudomonas syringae pv. phaseolicola, the causal agent of halo blight disease of bean (Phaseolus vulgaris) produces Phaseolotoxin (Nδ-(N′-sulpho-diaminophosphinyl)-L-ornithyl-alanyl-homoarginine) — a phytotoxic secondary metabolite — under laboratory conditions in a synthetic medium. Permeabilized (EDTA-treatment) and immobilized (Agar Agar) cells of the bacterium are capable of producing Phaseolotoxin. Therefore an “in situ” production of this microbial phytoeffective compound using immobilized and permeabilized cells of Pseudomonas syringae pv. phaseolicola is possible.