家蚕蛋白质组数据库草案

H. Kajiwara, K. Nakane, Jiang Piyang, A. Imamaki, Yoko Ito, Fumio Togasaki, Tsuyoshi Kotake, Hikari Murai, Masatoshi Nakamura, K. Mita, R. Nomura, Yuji Shimizu, Michihiko Shimomura, M. Ishizaka
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引用次数: 9

摘要

利用Make-2DDB II软件建立家蚕蛋白质组数据库(SPD)。本SPD包含家蚕七个主要组织的蛋白质组:中蚕腺、后蚕腺、中肠、脂肪体、血淋巴、卵巢和马尔比氏小管。采用MS/MS法对5龄第3天各组织蛋白进行鉴定,并利用2-DE法构建4龄第1天至成虫组织蛋白谱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Draft of silkworm proteome database
A silkworm (Bombyx mori) proteome database (SPD) was constructed using Make-2DDB II software. The present SPD contains the proteomes of the seven major tissues in the silkworm: the middle silkglands, posterior silkglands, midguts, fat bodies, hemolymph, ovaries, and Malpighian tubules. Proteins in each tissue at day 3 of the fifth instar were identified by MS/MS analysis and the protein profiles by 2-DE from day 1 of the fourth instar larva to the adult moth were constructed.
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