Toxic Effect of Methylation and Chlorpyrifos on Liver of Male Rabbits

The irrational use of organ phosphorus insecticides in Yemen play a crucial role in the occurrence of many diseases affecting plants, animals, and man. Methidathion (MD) and Chlorpyrifos (CF) are two of widely used organ phosphorus insecticides in agriculture. The purpose of the present study was to investigate the in vivo effect of (MD) and (CF) on lipid peroxidation (LPO), antioxidant defense system in the liver cells and liver function in male rabbits after orally administration a single dose of 1/8 of LD50 of (MD) and (CF) period of 20 days. Thirty healthy male rabbits weighting 1400-1600g., were divided into 3 groups with 10 animals in each, first group served as control animals, they received 3ml.of corn oil, while animals in second and third groups received 1/8 of LD50 of (MD) and (CF) respectively. The level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and gamma glutamyl transferase (γ-GT) in serum were analyzed, the level of (LPO), and methylglyoxal (MG), in liver homogenate were also studied. Results showed a significant (P<0.01) increase in all studied parameters in animals treated with (MD) and (CF) as compared to control animals. Our results indicated to the high toxicity of (MD) and (CF).