Determination of Pantothenyl Alcohol, Pantothenyl Ethyl Ether in Cosmetics by High Performance Liquid Chromatography.

A simple method for the simultaneous determination of pantothenyl alcohol (PA) and pantothenyl ethyl ether (PE) in cosmetics by high performance liquid chromatography has been developed. Samples (cosmetics) containing PA and PE were dissolved with chloroform (CHCl3). The samples including PA and PE were passed through Bondelute SI[○!R] cartrige. After washing the cartrige with CHCl3, PA and PE were eluted with methanol (MeOH). The optimum condition for the separation of PA and PE in cosmetics were as follows : column, TSK gel ODS 80 Ts (4.6 mm i.d.×150 mm) ; mobile phase, CH3CN-MeOH-H2O (15 : 10 : 75), 0.5 ml/min ; column temperature, 40°C ; detection wavelength, 210 nm. Recoveries of PA and PE from 7 cosmetics were more than 90%. Determination limits of PA and PE were 10 μg/g and 5 μg/g, respectively.