Effect of Polycaprolactone Scaffold Containing Myrtus Communis Extract and Silver Nanoparticles on the Burn Wound Infection Induced by Methicillinresistant Staphylococcus Aureus in Rats

Background and Objectives: Regarding the important role of Staphylococcus aureus in burn wound infection, the present study aims to investigate of the effect of Polycaprolactone (PCL) scaffold containing Myrtus communis extract and silver nanoparticles on the healing of burn wound infections induced by Methicillin-Resistant Staphylococcus Aureus (MRSA) in rats. Methods: In this experimental study, 42 male Wistar rats were divided into seven groups of 6. After anesthesia, second-degree burns on the back of the rats’ necks were created and MRSA was then injected into the wound area. The rats’ wounds were treated daily with nanofiber PCL containing Myrtus communis extract and silver nanoparticles. Antimicrobial properties, wound healing percentage, wound area, malondialdehyde and nitric oxide levels, and total antioxidant capacity were measured. Data were analyzed in GraphPad software using Tukey’s test and ANOVA. Results: The wound area measurement showed the better effects on would healing in the groups treated by PCL+Myrtus communis extract and PCL+Myrtus communis extract+silver nanoparticles compared other groups. In bacteriological studies, the groups treated by PCL+Myrtus communis extract and PCL+Myrtus communis extract+silver nanoparticle showed more reduced bacterial growth compared to the PCL alone and positive control groups. Moreover, biochemical tests showed a decrease in malondialdehyde and nitric oxide levels in rats treated by PCL+Myrtus communis extract compared to other groups. Conclusion: Myrtus communis extract and silver nanoparticles can cause changes in PCL properties, which have an important role in reducing the time required for wound healing and in preventing the progression of MRSA-induced wound infection