Effect of tumor necrosis factor-alpha on inflammatory reaction in fetal rat kidney after acute ischemia

Objective To study the effect of tumor necrosis factor alpha(TNF α)on inflammatory reaction in fetal rat kidney after acute ischemia.Methods By clamping one side of vessels supplying blood to uterus of Wistar rats of 21 gestational days, we made the models of intrauterine acute ischemia in different degrees and the reperfusion models in fetal rats. TNF α and myeloperoxidase (MPO) in fetal kidney tissue were detected by ELISA and biochemically respectively, and the renal histopathological changes were also observed.Results The activity of TNT α increased gradually with the extension of ischemia, especially after 35～45 minutes (P 0.05) .It rose progressively during reperfusion and was at its highest after 8 hours (P0.01) .It reached the level of the sham operation group after 30 hours reperfusion .The activity of MPO changed as that of TNF α . The activity of MPO reached the peak in 15 hours of reperfusion (P0.01) . Histological changes were most prominent after 15 hours reperfusion, including vascular congestion, tubular vacuolar degeneration or necrosis and neutrophil accumulation basement membrane breakdown, especially in proximal end of tubules.Conclusions There is inflammatory reaction in renal injury induced by intrauterine ischemia and reperfusion, TNF α may be one of important mediator and MPO can reflect the degree of inflammatory reaction.