{"title":"克拉多佛甲醇提取物对HT-29细胞株的体外抗癌活性研究","authors":"N. Theresita Shanthi, S. Murugesan, M. Kotteswari","doi":"10.9790/3008-1203075561","DOIUrl":null,"url":null,"abstract":"The anticancer activity of the marine alga Cladophora spp. against HT-29 colon cancer cell line was studied. In the present investigation crude extract of seaweed was prepared by using soxhlet apparatus. Methanol was used as solvent for extraction. In vitro anticancer activity of seaweed at various concentrations (7.8 to 1000 μg/mL) were studied against the chosen cell line by using MTT assay (3-(4, 5-dimethyl thiazol 2)2, 5-diphenyl tetrazolium bromide, a yellow tertrazol). The most potent antitumor activity has been shown at the concentration 7.8 μg/mL and 400 μg/mL of Cladaphora spp. extract on HT-29 colon cancer cell line.","PeriodicalId":14548,"journal":{"name":"IOSR Journal of Pharmacy and Biological Sciences","volume":"12 1","pages":"55-61"},"PeriodicalIF":0.0000,"publicationDate":"2017-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"In vitro anticancer activity of methanol extract of Cladophora spp. against HT-29 cell line\",\"authors\":\"N. Theresita Shanthi, S. Murugesan, M. Kotteswari\",\"doi\":\"10.9790/3008-1203075561\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The anticancer activity of the marine alga Cladophora spp. against HT-29 colon cancer cell line was studied. In the present investigation crude extract of seaweed was prepared by using soxhlet apparatus. Methanol was used as solvent for extraction. In vitro anticancer activity of seaweed at various concentrations (7.8 to 1000 μg/mL) were studied against the chosen cell line by using MTT assay (3-(4, 5-dimethyl thiazol 2)2, 5-diphenyl tetrazolium bromide, a yellow tertrazol). The most potent antitumor activity has been shown at the concentration 7.8 μg/mL and 400 μg/mL of Cladaphora spp. extract on HT-29 colon cancer cell line.\",\"PeriodicalId\":14548,\"journal\":{\"name\":\"IOSR Journal of Pharmacy and Biological Sciences\",\"volume\":\"12 1\",\"pages\":\"55-61\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"IOSR Journal of Pharmacy and Biological Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9790/3008-1203075561\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"IOSR Journal of Pharmacy and Biological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9790/3008-1203075561","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
In vitro anticancer activity of methanol extract of Cladophora spp. against HT-29 cell line
The anticancer activity of the marine alga Cladophora spp. against HT-29 colon cancer cell line was studied. In the present investigation crude extract of seaweed was prepared by using soxhlet apparatus. Methanol was used as solvent for extraction. In vitro anticancer activity of seaweed at various concentrations (7.8 to 1000 μg/mL) were studied against the chosen cell line by using MTT assay (3-(4, 5-dimethyl thiazol 2)2, 5-diphenyl tetrazolium bromide, a yellow tertrazol). The most potent antitumor activity has been shown at the concentration 7.8 μg/mL and 400 μg/mL of Cladaphora spp. extract on HT-29 colon cancer cell line.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
