Evaluation of remineralizing efficiency of two calcium-based non-fluoridated remineralizing agents for the prevention of white spot lesions (WSL): A comparative FESEM & EDS in-vitro study

This research work was designed to appraise and equate the remineralizing efficiency of two calcium-based phosphate delivery agents, Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP) and Calcium Sucrose Phosphate (CaSP) for preventing the development of White Spot Lesions (WSL) around the orthodontic brackets. Forty orthodontically removed premolar teeth were randomly allocated to Group 1(n=20) - (CPP-ACP) and Group 2(n=20) - (CaSP). All the specimens were subjected to FESEM and EDS analysis before the initial demineralization phase and after 14 days of the pH cycling phase, to observe and record changes in surface topography and mineral content (Ca/P ratio % wt.). Statistical data for the Intragroup comparison were analyzed by using Paired sample t-test, whereas for the Intergroup comparison, an Unpaired t-test was performed. Furthermore, the One-way ANOVA test was applied for comparing Intragroup data and the Post-hoc Tukey test was applied to compare Intergroup data. Group 1 samples showed an 11.3%increase in remineralization and Group 2 samples showed a 21.3%increase in remineralization, indicating a statistically significant (p < 0.05) 11% of greater remineralizing efficiency of CaSP. The use of Calcium Sucrose Phosphate (CaSP) tooth cream around the orthodontic brackets seems to produce significantly better remineralization effects.