Étude cinétique comparée de l'hydrolyse trypsique des lactoglobulines A et B fonction du pH

The tryptic hydrolysis of lactoglobulins A and B is followed for different pH values. The variations of the kinetic contants according to the pH, give us some information on the reaction mechanism. During the intermediary complex formation, the enzyme associating with the lactoglobulin (A or B form) induces the Tanford transformation of the substrate molecule with demasking of the anomalous carboxylic groups thereby facilitating their ionization.