{"title":"龙葵根中植物成分的抗氧化、脂质保护和抗菌活性研究","authors":"A. Pandey, Shashank Kumar","doi":"10.15866/IREBIC.V3I3.1557","DOIUrl":null,"url":null,"abstract":"Present study reports the biochemical and antibacterial activities of phytoconstituents present in roots of non toxic weed S. xanthocarpum. Total phenolic contents were quantified in different fractions. Ethanolic (ET) fraction was richest (194.44±0.41 mgPGE/g) followed by ethyl acetate (EA) and aqueous (AQ) fractions (115±0.25 and 88.75± mgPGE/g, respectively). Antioxidant activity was evaluated using reducing power assay (RPA) while lipo-protective activity was determined by lipid peroxidation inhibition (LPOI)/TBARS assays in rat kidney tissue. ET, AQ and HX (hexane) fractions demonstrated significant reducing power at 1000 µg/ml concentration showing dose dependent response in the range 200-1000 µg/ml. ET and AQ fractions exhibited 52% and 45% LPOI, respectively in TBARS assay. Positive correlation was observed between total phenol and RPA (r2=0.399) as well as %LPOI (r2=0.540). Similarly relationship between RPA and %LPOI was also found to be positive (r2=0.379). Pathogenic bacteria Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Klebsiella pneumonia and Staphylococcus aureus exhibited resistance against AQ fraction in disc diffusion assay","PeriodicalId":14377,"journal":{"name":"International Review of Biophysical Chemistry","volume":"1 1","pages":"42-47"},"PeriodicalIF":0.0000,"publicationDate":"2012-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"56","resultStr":"{\"title\":\"Antioxidant, Lipo-Protective and Antibacterial Activities of Phytoconstituents Present in Solanum Xanthocarpum Root\",\"authors\":\"A. Pandey, Shashank Kumar\",\"doi\":\"10.15866/IREBIC.V3I3.1557\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Present study reports the biochemical and antibacterial activities of phytoconstituents present in roots of non toxic weed S. xanthocarpum. Total phenolic contents were quantified in different fractions. Ethanolic (ET) fraction was richest (194.44±0.41 mgPGE/g) followed by ethyl acetate (EA) and aqueous (AQ) fractions (115±0.25 and 88.75± mgPGE/g, respectively). Antioxidant activity was evaluated using reducing power assay (RPA) while lipo-protective activity was determined by lipid peroxidation inhibition (LPOI)/TBARS assays in rat kidney tissue. ET, AQ and HX (hexane) fractions demonstrated significant reducing power at 1000 µg/ml concentration showing dose dependent response in the range 200-1000 µg/ml. ET and AQ fractions exhibited 52% and 45% LPOI, respectively in TBARS assay. Positive correlation was observed between total phenol and RPA (r2=0.399) as well as %LPOI (r2=0.540). Similarly relationship between RPA and %LPOI was also found to be positive (r2=0.379). Pathogenic bacteria Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Klebsiella pneumonia and Staphylococcus aureus exhibited resistance against AQ fraction in disc diffusion assay\",\"PeriodicalId\":14377,\"journal\":{\"name\":\"International Review of Biophysical Chemistry\",\"volume\":\"1 1\",\"pages\":\"42-47\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"56\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Review of Biophysical Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15866/IREBIC.V3I3.1557\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Review of Biophysical Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15866/IREBIC.V3I3.1557","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Antioxidant, Lipo-Protective and Antibacterial Activities of Phytoconstituents Present in Solanum Xanthocarpum Root
Present study reports the biochemical and antibacterial activities of phytoconstituents present in roots of non toxic weed S. xanthocarpum. Total phenolic contents were quantified in different fractions. Ethanolic (ET) fraction was richest (194.44±0.41 mgPGE/g) followed by ethyl acetate (EA) and aqueous (AQ) fractions (115±0.25 and 88.75± mgPGE/g, respectively). Antioxidant activity was evaluated using reducing power assay (RPA) while lipo-protective activity was determined by lipid peroxidation inhibition (LPOI)/TBARS assays in rat kidney tissue. ET, AQ and HX (hexane) fractions demonstrated significant reducing power at 1000 µg/ml concentration showing dose dependent response in the range 200-1000 µg/ml. ET and AQ fractions exhibited 52% and 45% LPOI, respectively in TBARS assay. Positive correlation was observed between total phenol and RPA (r2=0.399) as well as %LPOI (r2=0.540). Similarly relationship between RPA and %LPOI was also found to be positive (r2=0.379). Pathogenic bacteria Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Klebsiella pneumonia and Staphylococcus aureus exhibited resistance against AQ fraction in disc diffusion assay
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
