固氮剂Paenibacillus sp. 1-49培养基优化。

微体古生物学报 Pub Date : 2016-09-01 DOI:10.13343/J.CNKI.WSXB.20150538

Zhiping Deng, Hao Chen, San-feng Chen

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引用次数: 0

摘要

目的拟芽孢杆菌(paenibacillus sp. 1-49)是一种具有肥料潜力的固氮菌。但无论在矿物培养基还是在富质培养基中，其生长均较差(OD600≤1)。为获得高产生物量，对芽孢杆菌1-49的发酵培养基进行了优化。方法采用响应面法中的plackett - burman设计和中心复合设计优化培养基组成。结果最佳发酵培养基:(每升)蔗糖36.22 g、色氨酸5.31 g、酵母膏10.92 g、MgSO4 0.51 g、NaCl 3.5 g、Na2MoO4 0.02 g、FeSO4 0.02 g。摇瓶发酵的OD600最大值为10.280±0.009，达到预测值的94.6%。结论采用响应面法对芽孢杆菌1-49的发酵培养基进行了优化。我们的研究结果将为该菌株和其他类芽孢杆菌的大规模发酵提供参考。

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Medium optimization for nitrogen fixer Paenibacillus sp. 1-49.

Objective Paenibacillus sp. 1-49 is a nitrogen-fixing bacterium that has the potential as a fertilizer. However, it grows poorly (OD600≤1) in both mineral medium and rich medium. To achieve high-yield biomass, we optimized fermentation medium for Paenibacillus sp. 1-49. Methods Plackett-Burman Design and Central Composite Design in response surface methodology were used to optimize medium composition. Results The optimal fermentation medium contained:(per liter) 36.22 g Sucrose, 5.31 g Tryptone, 10.92 g Yeast Extract, 0.51 g MgSO4, 3.5 g NaCl, 0.02 g Na2MoO4 and 0.02 g FeSO4. The maximum OD600 of 10.280±0.009 was obtained in shake flask fermentation, reached 94.6% of the predicted value. Conclusion We succeeded in using a response surface methodology to optimize the fermentation medium for Paenibacillus sp. 1-49. Our results will be useful for largescale fermentation of this strain and other Paenibacillus spp.

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微体古生物学报

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