Evaluating genetic diversity and chemical substances of Disporopsis longifolia craib samples collected in Vietnam

Disporopsis longifolia Craib is a valuable medicinal plant and has been recorded in the Red Data Book in many countries, including Vietnam. Fifteen samples of Disporopsis (leaves and tubers) were collected in the fifteen different communes of five districts in Ha Giang province. The samples were sequenced by amplifying the trnH-psbA locus in the chloroplast. All sequence data were compared with the reference genes published in NCBI. The results showed a significantly high genetic similarity among the fifteen samples, ranging from 98.5%-100%. The genetic similarity factors also ranged from 96.67- 97.48% with reference sample KJ745836.1- D.longifolia, which had been published data. Fifteen samples and references were divided into two main groups. This result shows a close relationship between the studied samples of Disporopsis with the genus D.longifolia . The main chemical components of this species were initially identified, including polysaccha-86 rides, saponins and amino acids. The total polysaccharide content calculated by glucose ranges from 6.94% to 24.0%. This is considerably useful for the conservation, exploitation and effective use of this precious genetic resource in Vietnam.