{"title":"废酿酒酵母RNA提取中蛋白质的去除","authors":"Lt, strong gt, 王知力 lt, 郑飞云, 李崎, 顾国贤","doi":"10.3969/J.ISSN.0254-5071.2006.03.009","DOIUrl":null,"url":null,"abstract":"Three methods of removing proteins in RNA were discussed in this paper.The best way was to add 40 U neutral protease per gram dry yeast,and to compare the effects of protein removal to the loss of RNA.The extraction yield of this crude RNA product was 5.75% while the removal ratio of protein was 96.6%.","PeriodicalId":9837,"journal":{"name":"China Brewing","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Removal of protein in RNA extraction of waste brewing yeast\",\"authors\":\"Lt, strong gt, 王知力 lt, 郑飞云, 李崎, 顾国贤\",\"doi\":\"10.3969/J.ISSN.0254-5071.2006.03.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Three methods of removing proteins in RNA were discussed in this paper.The best way was to add 40 U neutral protease per gram dry yeast,and to compare the effects of protein removal to the loss of RNA.The extraction yield of this crude RNA product was 5.75% while the removal ratio of protein was 96.6%.\",\"PeriodicalId\":9837,\"journal\":{\"name\":\"China Brewing\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"China Brewing\",\"FirstCategoryId\":\"1087\",\"ListUrlMain\":\"https://doi.org/10.3969/J.ISSN.0254-5071.2006.03.009\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"China Brewing","FirstCategoryId":"1087","ListUrlMain":"https://doi.org/10.3969/J.ISSN.0254-5071.2006.03.009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Removal of protein in RNA extraction of waste brewing yeast
Three methods of removing proteins in RNA were discussed in this paper.The best way was to add 40 U neutral protease per gram dry yeast,and to compare the effects of protein removal to the loss of RNA.The extraction yield of this crude RNA product was 5.75% while the removal ratio of protein was 96.6%.