利用微卫星标记分析巴拉迪鸡品种与sasso和Ross品系的遗传多样性

M. El-sayed, S. Mekky, H. Assi, H. Zaky
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引用次数: 0

摘要

采用12个微卫星标记对2个商业鸡种Sasso (n=20)、Ross (n=20)和1个地方鸡种Baladi (n=20)的60只个体进行扩增。在3个鸡群体的12个位点中,共发现了96个等位基因,平均每个位点8个等位基因。ADL0315位点共有15个等位基因;MCW0210位点仅有3个等位基因。在特定等位基因上,总体位点(12个微卫星位点)在3个群体中发现了96个等位基因中的39个(40.63%)。在Baladi品种中发现了20个不同的等位基因,在Sasso品种中发现了5个等位基因,而在Ross品种中发现了14个等位基因。大部分等位基因频率分布在低频,但14个等位基因频率高于0.5。Baladi品系的PIC和He平均值分别为0.679和0.70,Sasso品系的PIC和He平均值最低,分别为0.588和0.61。除MCW0210有1个标记具有较好的信息外,巴拉地鸡品种的PIC值均超过0.5。此外,除了MCW0210、ADL0037、MCW0206、MCW0002和ADL0158 5个标记具有较高的信息标记外,Sasso鸡株的PIC值均超过0.5。结果表明,除MCW0210、ADL0037和MCW0016 3个标记具有较高的信息标记外,罗斯鸡菌株的PIC值均超过0.5。Baladi品系和Sasso品系的IC均值分别为0.23和0.28,表明近交水平高,种群变异性低。而罗斯品种的平均值为0.1,表明该品种的变异性很高。这些发现可以归因于罗斯品种比巴拉迪品种和萨索品种更近的分化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
GENETIC DIVERSITY BETWEEN BALADI CHICKEN BREED AND SASSO AND ROSS STRAINS USING MICROSATELLITE MARKERS
: Sixty individuals from two commercial strains Sasso (n=20), Ross (n=20), and one local chicken breed Baladi (n=20) were amplified by 12 microsatellite markers. Over 12 loci in three chicken populations, we found a total of 96 alleles with an average of 8 alleles per locus. There were 15 alleles in locus ADL0315; and only 3 alleles in locus MCW0210. In specific alleles, overall loci (12 microsatellite loci) found 39 out of 96 alleles (40.63 %) with three populations. Twenty distinct alleles were found for the Baladi breed and 5 alleles for the Sasso strain, while 14 were found for the Ross strain. Most frequencies of alleles were distributed with low frequency, but the 14 allele frequency was higher than 0.5. The PIC and He averages in the Baladi breed were 0.679 and 0.70, respectively, while those in the Sasso strain were 0.588 and 0.61, respectively, which were the lowest. In Baladi chicken breed has highly informative PIC values exceed 0.5 except one marker of MCW0210 had reasonably informative markers. Also, in Sasso chicken strain has highly informative PIC values exceed 0.5 except five markers of MCW0210, ADL0037, MCW0206, MCW0002, and ADL0158 had reasonably informative markers. Finally, in Ross chicken strain has highly informative PIC values exceed 0.5 except three markers of MCW0210, ADL0037 and MCW0016 had reasonably informative markers. The mean values of IC obtained estimate 0.23 and 0.28 for Baladi breed and Sasso strain respectively, indicating the high level of inbreeding indicating low variability in their populations. While the mean value of 0.1 for the Ross breed indicating high variability in this breed. These findings could be attributed to a more recent divergence of Ross than Baladi breed and Sasso strain.
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