陆地棉微管蛋白基因家族的鉴定与表达分析

IF 3.1 4区 生物学 Q2 PLANT SCIENCES
Bao-jun Chen, Junjie Zhao, Guoyong Fu, Xinxin Pei, Zhaoe Pan, Hongge Li, Haris Ahmed, Shoupu He, X. Du
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引用次数: 4

摘要

棉纤维是种子表皮的单细胞延伸,是研究细胞骨架的模型组织。微管蛋白基因在合成微管(MT)中起着关键作用,微管是细胞骨架的核心元素。然而,关于棉花微管蛋白基因家族的系统表征,目前还缺乏研究。因此,毛棉微管蛋白基因的鉴定和刻画可为棉花育种分子调控提供关键靶点。结果本研究对不同植物的微管蛋白基因进行了分析,鉴定出98个微管蛋白基因。系统发育分析表明,微管蛋白家族基因可分为3个亚科。与γ-微管蛋白基因相比,α-、β-微管蛋白基因的蛋白基序和基因结构更为保守。大多数微管蛋白基因位于染色体的近端。转录组和qRT-PCR分析显示,12个α-微管蛋白和7个β-微管蛋白基因在纤维发育的不同阶段特异性表达。然而,Gh。A03G027200, Gh。D03G169300;A11G258900在J02508和ZRI015不同纤维发育阶段的表达模式存在差异。结论本研究的进化分析表明,微管蛋白基因可分为三个支系。其遗传结构和分子功能在不同植物中高度保守。三个候选基因,Gh。A03G027200, Gh。D03G169300;A11G258900可能在纤维发育过程中起着补充纤维长度和强度的关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification and expression analysis of Tubulin gene family in upland cotton
Background Cotton fibers are single-celled extensions of the seed epidermis, a model tissue for studying cytoskeleton. Tubulin genes play a critical role in synthesizing the microtubules (MT) as a core element of the cytoskeleton. However, there is a lack of studies concerning the systematic characterization of the tubulin gene family in cotton. Therefore, the identification and portrayal of G. hirsutum tubulin genes can provide key targets for molecular manipulation in cotton breeding. Result In this study, we investigated all tubulin genes from different plant species and identified 98 tubulin genes in G. hirsutum . Phylogenetic analysis showed that tubulin family genes were classified into three subfamilies. The protein motifs and gene structure of α-, β- tubulin genes are more conserved compared with γ- tubulin genes. Most tubulin genes are located at the proximate ends of the chromosomes. Spatiotemporal expression pattern by transcriptome and qRT-PCR analysis revealed that 12 α- tubulin and 7 β- tubulin genes are specifically expressed during different fiber development stages. However, Gh.A03G027200 , Gh.D03G169300, and Gh.A11G258900 had differential expression patterns at distinct stages of fiber development in varieties J02508 and ZRI015. Conclusion In this study, the evolutionary analysis showed that the tubulin genes were divided into three clades. The genetic structures and molecular functions were highly conserved in different plants. Three candidate genes, Gh.A03G027200 , Gh.D03G169300, and Gh.A11G258900 may play a key role during fiber development complementing fiber length and strength.
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来源期刊
Journal of Cotton Research
Journal of Cotton Research PLANT SCIENCES-
CiteScore
5.20
自引率
3.80%
发文量
99
审稿时长
15 weeks
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