单链、双链和g -四重奏核酸与单链dna结合蛋白复合物的CD。

Enantiomer Pub Date : 2002-03-01 DOI:10.1080/10242430212192
D. Gray, C. Gray, T. Mou, J. Wen
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引用次数: 16

摘要

我们回顾了细菌病毒Ff群的单链DNA结合蛋白g5p的CD研究。g5p的CD光谱在229 nm处以酪氨酸La带为主,5种蛋白酪氨酸都参与其中。当g5p与核酸结合时,La带的正电性大大降低。突变蛋白的CD光谱鉴定出一个单独的酪氨酸Y34,这是造成CD扰动的主要原因。在>250 nm处,可以监测到核酸在g5p结合过程中的CD扰动,并且CD滴定鉴定出生理离子强度(0.2 M NaCl)下g5p的两种不同结合模式。SELEX选择g5p优先结合的序列,得到了与端粒序列密切相关的富含g的序列,并具有g -四联体的CD特性。CD光谱显示,假定的g -四重体形式在饱和的g5px DNA复合物中保持。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CD of single-stranded, double-stranded, and G-quartet nucleic acids in complexes with a single-stranded DNA-binding protein.
We review CD studies of a single-stranded DNA binding protein, g5p, of the Ff group of bacterial viruses. The CD spectrum of the g5p is dominated by a positive tyrosine La band at 229 nm, to which all five of the protein tyrosines contribute. The La band becomes much less positive upon binding of g5p to nucleic acids. CD spectra of mutant proteins identified a single tyrosine, Y34, that is largely responsible for this CD perturbation. At >250 nm, CD perturbations of nucleic acids can be monitored during g5p binding, and CD titrations have identified two distinct modes of binding of the g5p at physiological ionic strength (0.2 M NaCl). SELEX selection of sequences bound preferentially by g5p yielded a G-rich sequence that is closely related to telomere sequences and has CD properties of a G-tetraplex. CD spectroscopy showed that the presumed G-quadruplex form is maintained within saturated g5p x DNA complexes.
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