漆酶的生产和染料的脱色:田口和Box-Behnken方法的应用

IF 0.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI:10.5505/TJB.2014.62533

S. Gedikli, Yeliz Buruk, E. Apohan, A. Çabuk, O. Yesilada, N. Burnak

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引用次数: 4

摘要

目的:研究色曲霉在深层发酵条件下漆酶的产生。然后，采用Box-Behnken法优化漆酶对染料的脱色效果。方法:采用田口法确定高产漆酶的最佳培养条件。试验采用L27(35)正交设计，以葡萄糖、酵母浸膏、cuso4、接种量和pH为5个因素，进行3个水平的试验设计。然后，采用Box-Behnken方法对活性蓝49粗漆酶高脱色活性的最佳条件进行了研究。结果:葡萄糖用量为2 g L -1，葡萄糖用量为5 g L -1，是产生大量漆酶的最佳培养条件

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Laccase production and dye decolorization by Trametes versicolor: application of Taguchi and Box-Behnken Methodologies

Objective: The aim of this study was to investigate the laccase production of Trametes versicolor under submerged fermentation condition. Then, dye decolorization by laccase was optimized using Box-Behnken methodology. Methods: The optimal culture conditions for producing high amount of laccase were determined using Taguchi methodology. The experiments were designed with five factors (glucose, yeast extract, CuSO 4 , inoculum size and pH) at three levels with orthogonal array layout of L27 (3 5 ). Then, the optimum conditions for high decolorization activity of Reactive Blue 49 by obtained crude laccase were also investigated using Box-Behnken methodology. Results: The optimum culture conditions for production of high amounts of laccase were detected as 2 g L -1 of glucose, 5 g L -1

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来源期刊

Turkish Journal of Biochemistry-turk Biyokimya Dergisi 生物-生化与分子生物学

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Turkish Journal of Biochemistry (TJB), official journal of Turkish Biochemical Society, is issued electronically every 2 months. The main aim of the journal is to support the research and publishing culture by ensuring that every published manuscript has an added value and thus providing international acceptance of the “readability” of the manuscripts published in the journal.