印尼日惹Krakal沿海地区海水中异养细菌产生的类胡萝卜素色素的抗菌活性

Q3 Environmental Science
Rina Septriani Sidin, E. Retnaningrum
{"title":"印尼日惹Krakal沿海地区海水中异养细菌产生的类胡萝卜素色素的抗菌活性","authors":"Rina Septriani Sidin, E. Retnaningrum","doi":"10.15578/squalen.648","DOIUrl":null,"url":null,"abstract":"Investigating natural pigments resulting from marine bacterial secondary metabolites is important because of their broad benefits in the food, pharmaceutical, cosmetic, and textile industries. In this study, 52 isolates of pigmented bacteria were isolated from seawater in the Krakal coastal area, Yogyakarta, Indonesia. Seven bacterial isolates showed high pigment stability. Profiling of pigment extracts using a mixture of acetone and methanol with UV–Vis spectrophotometric reading showed that the compounds produced were carotenoids. The highest total carotenoid content with UV–Vis spectrophotometric reading at 480 nm was produced by the Kral-3 isolate (1800 mg/g), followed by Kral-15 (1698.9 mg/g) and Kral-25 (797.2 mg/g) isolates. Analysis of the antibacterial activity of the carotenoid extracts of Kral-3, Kral-15, and Kral-25 isolates using the agar well diffusion method revealed a very strong inhibition score against Escherichia coli. In addition, the carotenoid extract of the Kral-3 isolate showed a strong inhibition score against Staphylococcus aureus, whereas the Kral-15 and Kral-25 isolates showed moderate inhibition scores. Based on the 16S rRNA gene analysis, the Kral-3, Kral-15, and Kral-25 isolates were identified as Kocuria rhizophilla, Calidifontibacter sp., and Rhodococcus ruber, respectively. Based on the findings, the novel bacterial strains are a potential bioresource for the commercial production of natural carotenoids.","PeriodicalId":21935,"journal":{"name":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antibacterial Activity of Carotenoid Pigments Produced by Heterotrophic Bacteria from Seawater in Krakal Coastal Area, Yogyakarta, Indonesia\",\"authors\":\"Rina Septriani Sidin, E. Retnaningrum\",\"doi\":\"10.15578/squalen.648\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Investigating natural pigments resulting from marine bacterial secondary metabolites is important because of their broad benefits in the food, pharmaceutical, cosmetic, and textile industries. In this study, 52 isolates of pigmented bacteria were isolated from seawater in the Krakal coastal area, Yogyakarta, Indonesia. Seven bacterial isolates showed high pigment stability. Profiling of pigment extracts using a mixture of acetone and methanol with UV–Vis spectrophotometric reading showed that the compounds produced were carotenoids. The highest total carotenoid content with UV–Vis spectrophotometric reading at 480 nm was produced by the Kral-3 isolate (1800 mg/g), followed by Kral-15 (1698.9 mg/g) and Kral-25 (797.2 mg/g) isolates. Analysis of the antibacterial activity of the carotenoid extracts of Kral-3, Kral-15, and Kral-25 isolates using the agar well diffusion method revealed a very strong inhibition score against Escherichia coli. In addition, the carotenoid extract of the Kral-3 isolate showed a strong inhibition score against Staphylococcus aureus, whereas the Kral-15 and Kral-25 isolates showed moderate inhibition scores. Based on the 16S rRNA gene analysis, the Kral-3, Kral-15, and Kral-25 isolates were identified as Kocuria rhizophilla, Calidifontibacter sp., and Rhodococcus ruber, respectively. Based on the findings, the novel bacterial strains are a potential bioresource for the commercial production of natural carotenoids.\",\"PeriodicalId\":21935,\"journal\":{\"name\":\"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-08-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15578/squalen.648\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Environmental Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15578/squalen.648","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Environmental Science","Score":null,"Total":0}
引用次数: 0

摘要

研究由海洋细菌次生代谢产物产生的天然色素是很重要的,因为它们在食品、制药、化妆品和纺织工业中具有广泛的益处。本研究从印度尼西亚日惹市Krakal沿海地区的海水中分离出52株色素细菌。7株分离菌表现出较高的色素稳定性。用丙酮和甲醇的混合物对色素提取物进行紫外-可见分光光度分析,所得化合物为类胡萝卜素。在480 nm处,Kral-3 (1800 mg/g)的总类胡萝卜素含量最高,其次是Kral-15 (1698.9 mg/g)和Kral-25 (797.2 mg/g)。用琼脂孔扩散法对Kral-3、Kral-15和Kral-25分离物的类胡萝卜素提取物进行抑菌活性分析,发现其对大肠杆菌有很强的抑制作用。此外,Kral-3分离物的类胡萝卜素提取物对金黄色葡萄球菌具有较强的抑制评分,而Kral-15和Kral-25分离物的抑制评分为中等。根据16S rRNA基因分析,分离株Kral-3、Kral-15和Kral-25分别为Kocuria rhizophilla、Calidifontibacter sp.和Rhodococcus ruber。基于这些发现,新的细菌菌株是一种潜在的生物资源,用于商业生产天然类胡萝卜素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antibacterial Activity of Carotenoid Pigments Produced by Heterotrophic Bacteria from Seawater in Krakal Coastal Area, Yogyakarta, Indonesia
Investigating natural pigments resulting from marine bacterial secondary metabolites is important because of their broad benefits in the food, pharmaceutical, cosmetic, and textile industries. In this study, 52 isolates of pigmented bacteria were isolated from seawater in the Krakal coastal area, Yogyakarta, Indonesia. Seven bacterial isolates showed high pigment stability. Profiling of pigment extracts using a mixture of acetone and methanol with UV–Vis spectrophotometric reading showed that the compounds produced were carotenoids. The highest total carotenoid content with UV–Vis spectrophotometric reading at 480 nm was produced by the Kral-3 isolate (1800 mg/g), followed by Kral-15 (1698.9 mg/g) and Kral-25 (797.2 mg/g) isolates. Analysis of the antibacterial activity of the carotenoid extracts of Kral-3, Kral-15, and Kral-25 isolates using the agar well diffusion method revealed a very strong inhibition score against Escherichia coli. In addition, the carotenoid extract of the Kral-3 isolate showed a strong inhibition score against Staphylococcus aureus, whereas the Kral-15 and Kral-25 isolates showed moderate inhibition scores. Based on the 16S rRNA gene analysis, the Kral-3, Kral-15, and Kral-25 isolates were identified as Kocuria rhizophilla, Calidifontibacter sp., and Rhodococcus ruber, respectively. Based on the findings, the novel bacterial strains are a potential bioresource for the commercial production of natural carotenoids.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
16 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信