可透析白细胞提取物(DLE)和肌苷对受刺激淋巴细胞的影响。

F. Komatsu
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引用次数: 1

摘要

观察可透析白细胞提取物(DLE)对淋巴细胞的影响。粗DLE抑制pha刺激淋巴细胞的形成和白血病细胞系的增殖,但增强成纤维细胞的增殖。一定比例的DLE使受刺激淋巴细胞的ATP水平在孵育24小时后升高。进一步纯化该组分,并从中洗脱肌苷。肌苷能提高ATP水平,显著促进成纤维细胞和白血病细胞系淋巴细胞的形成和增殖。提示当DLE对淋巴细胞有一定作用时,其所含肌苷可能发挥重要作用。肌苷的作用被其他物质所抑制,尤其是脱氧核苷酸。这些物质抑制淋巴细胞的形成和白血病细胞系的增殖。为什么成纤维细胞不受它们的抑制还有待确定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of dialyzable leukocyte extracts (DLE) and inosine on stimulated lymphocytes.
The effects of dialyzable leukocyte extracts (DLE) on the lymphocytes were examined. The crude DLE suppressed the blast-formation of the PHA-stimulated lymphocytes and proliferation of the leukemic cell-lines but enhanced that of the fibroblastic cells. A certain fraction of the DLE caused the ATP level of the stimulated lymphocytes to rise, after incubation for 24 hours. The fraction was further purified and the inosine eluted from it. The inosine increased the ATP level and markedly enhanced the lymphocyte blast-formation and proliferation of the fibroblastic cells and leukemic cell-lines. It is suggested that when the DLE show some effects on the lymphocytes, the inosine contained in the DLE may show important effects. The effect of inosine was inhibited by other substances contained in the DLE, especially deoxynucleotides. These substances suppressed the blast-formation of the lymphocytes and the proliferation of the leukemic-cell lines. It remains to determine why the fibroblastic cells were not suppressed by them.
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