利用染色体行走定位水稻耐淹性相关数量性状位点。

DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes Pub Date : 2001-01-01 DOI:10.1093/DNARES/8.4.163

W. Kamolsukyunyong, V. Ruanjaichon, M. Siangliw, S. Kawasaki, T. Sasaki, A. Vanavichit, S. Tragoonrung

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引用次数: 25

摘要

淹水耐受性的主要QTL位于侧翼RFLP标记间的5.9 cM区间。为了缩小这一区域，利用YAC和BAC克隆构建了物理地图。在该区域鉴定出一个400 kb的YAC，并利用其末端片段筛选水稻BAC文库。通过染色体行走，24个阳性BAC克隆在连锁rflp标记R1164和RZ698周围形成两个contigs。利用1个YAC端、6个BAC端和3个RFLP标记，构建了水稻9号染色体S10709-RZ698位点6.8 cm区间的精细图谱。耐淹性和相关性状位于bac末端标记180D1R和RFLP标记R1164周围的一个小而明确的区域。该区域的物理与图谱距离比小至172.5 kb/cM，表明该区域是水稻基因组重组的热点区域。

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Mapping of quantitative trait locus related to submergence tolerance in rice with aid of chromosome walking.

The major QTL for submergence tolerance was locate in the 5.9 cM interval between flanking RFLP markers. To narrow down this region, a physical map was constructed using YAC and BAC clones. A 400-kb YAC was identified in this region and later its end fragments were used to screen a rice BAC library. Through chromosome walking, 24 positive BAC clones formed two contigs around linked-RFLP markers, R1164 and RZ698. Using one YAC end, six BAC ends and three RFLP markers, a fine-scale map was constructed of the 6.8-cM interval of S10709-RZ698 on rice chromosome 9. The submergence tolerance and related trait were located in a small, well-defined region around BAC-end marker 180D1R and RFLP marker R1164. The physical-to-map distance ratio in this region is as small as 172.5 kb/cM, showing that this region is a hot spot for recombination in the rice genome.

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DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

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