用微卫星分析鉴定濒临灭绝的青岭短柄霉(鲑科)亲本

IF 1.3 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Aquatic Biology Pub Date : 2017-06-19 DOI:10.3354/AB00675

J. Shao, W. Luo, Q. Wei, Feng Wang, Wei Guo, H. Ye, Zhipeng Chu, Jinming Wu, Shuhuan Zhang

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引用次数: 2

摘要

本研究利用微卫星标记对濒危鲑鱼Brachy mystax lenok tsinlingensis Li, 1966进行亲本鉴定。计算机模拟结果表明，当没有亲本信息时，排除概率为23.6 ~ 45.1%，当只有一个亲本信息时，排除概率为25.9 ~ 52.4%;8个基因座的排除率分别为97.7和98.4%。然而，在已知亲本和子代信息的育种实验中，91.28%的后代被排他性地分配给正确的亲本。我们的数据表明，基于微卫星的亲子鉴定是一种可靠的方法，可以获得有关家谱关系的信息，从而有利于该物种的遗传保护。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Assignment of parentage by microsatellite analysis in the endangered Brachymystax lenok tsinlingensis (Salmonidae)

We evaluated the use of microsatellite markers to identify parentage in Brachy mystax lenok tsinlingensis Li, 1966, an endangered salmonid. Computer simulations showed that when no parent information was available, or when information was only available from 1 parent, then exclusion probabilities ranged from 23.6 to 45.1% and from 25.9 to 52.4%, respectively; combined exclusion probabilities for the 8 loci investigated were 97.7 and 98.4%, respectively. However, a breeding experiment with known parental and filial information resulted in 91.28% of progeny being exclusively assigned to their correct parent pair. Our data demonstrate that microsatellitebased parentage assignment is a reliable means with which to obtain information pertaining to genealogical relationships and could therefore benefit genetic conservation of this species.

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来源期刊

Aquatic Biology 生物-海洋与淡水生物学

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： AB publishes rigorously refereed and carefully selected Feature Articles, Research Articles, Reviews and Notes, as well as Comments/Reply Comments (for details see MEPS 228:1), Theme Sections, Opinion Pieces (previously called ''As I See It'') (for details consult the Guidelines for Authors) concerned with the biology, physiology, biochemistry and genetics (including the ’omics‘) of all aquatic organisms under laboratory and field conditions, and at all levels of organisation and investigation. Areas covered include: -Biological aspects of biota: Evolution and speciation; life histories; biodiversity, biogeography and phylogeography; population genetics; biological connectedness between marine and freshwater biota; paleobiology of aquatic environments; invasive species. -Biochemical and physiological aspects of aquatic life; synthesis and conversion of organic matter (mechanisms of auto- and heterotrophy, digestion, respiration, nutrition); thermo-, ion, osmo- and volume-regulation; stress and stress resistance; metabolism and energy budgets; non-genetic and genetic adaptation. -Species interactions: Environment–organism and organism–organism interrelationships; predation: defenses (physical and chemical); symbioses. -Molecular biology of aquatic life. -Behavior: Orientation in space and time; migrations; feeding and reproductive behavior; agonistic behavior. -Toxicology and water-quality effects on organisms; anthropogenic impacts on aquatic biota (e.g. pollution, fisheries); stream regulation and restoration. -Theoretical biology: mathematical modelling of biological processes and species interactions. -Methodology and equipment employed in aquatic biological research; underwater exploration and experimentation. -Exploitation of aquatic biota: Fisheries; cultivation of aquatic organisms: use, management, protection and conservation of living aquatic resources. -Reproduction and development in marine, brackish and freshwater organisms