沉淀物分离方法对生物测定结果的影响

Environmental Toxicology & Water Quality Pub Date : 1994-05-01 DOI:10.1002/TOX.2530090211

K. Ho, L. Mills, C. Mueller, S. Anderson, A. Malcolm

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引用次数: 20

摘要

采用MicrotoxTM、MutatoxTM、姐妹染色单体交换(SCE)和代谢合作(metabolic cooperation)四种生物测定方法对硅胶柱层析和酸碱分馏两种不同方法分离的海洋沉积物提取物进行了分析。结果表明，对同一种沉积物，不同的分馏方法可得到不同的分馏结论。本研究还进一步评价了新的、诱变的、细菌生物测定的Mutatox。突变通常与SCE和鼠伤寒沙门菌检测相关。这种快速、操作简单的生物测定法有潜力作为一种筛选生物测定法来检测基因毒性物质。©1994 by John Wiley & Sons, Inc.。

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The influence of sediment extract fractionation methods on bioassay results

Four bioassays [MicrotoxTM, MutatoxTM, sister chromatid exchange (SCE), and metabolic cooperation] were used to analyze marine sediment extracts fractionated by two different methods: silica gel column chromatography and acid–base fractionation. Results indicated that a sediment extract fractionated with different methods can lead to different conclusions about the same sediment. This research also further evaluated the new, mutagenic, bacterial bioassay Mutatox. Mutatox generally correlated with SCE and the Salmonella typhimurium assays. This rapid, operationally simple bioassay has potential as a screening bioassay to detect genotoxic agents. © 1994 by John Wiley & Sons, Inc..

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Environmental Toxicology & Water Quality

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