{"title":"黑麦与黑麦杂种体细胞无性系的染色体不稳定性","authors":"G. Fedak, J. Grainger","doi":"10.1139/G86-090","DOIUrl":null,"url":null,"abstract":"Immature inflorescence culture and subsequent plant regeneration was practiced for four successive cycles using a Triticum crassum × Hordeum vulgare hybrid cultured on Kao's medium supplemented with 2,4-dichlorophenoxyacetic acid (5 mg/mL). In one line, chromosomal mixoploidy was observed among both mitotic and meiotic cells. Variation in chromosome number of 20 to 98 was observed in mitotic and 14 to 68 among meiocytes in the first cycle regenerants. The range in chromosome number decreased in subsequent regeneration cycles. Fragmented chromosomes were observed at low frequencies in both groups of cells. The high frequency of univalents at meiosis was attributed to possible elimination of chromosomes carrying meiotic pairing control genes.Key words: somaclonal variation, hybrids (intergeneric), tissue culture, chromosomal mixoploidy.","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"18 1","pages":"618-623"},"PeriodicalIF":0.0000,"publicationDate":"1986-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"16","resultStr":"{\"title\":\"Chromosome instability in somaclones of a Triticum crassum × Hordeum vulgare hybrid\",\"authors\":\"G. Fedak, J. Grainger\",\"doi\":\"10.1139/G86-090\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Immature inflorescence culture and subsequent plant regeneration was practiced for four successive cycles using a Triticum crassum × Hordeum vulgare hybrid cultured on Kao's medium supplemented with 2,4-dichlorophenoxyacetic acid (5 mg/mL). In one line, chromosomal mixoploidy was observed among both mitotic and meiotic cells. Variation in chromosome number of 20 to 98 was observed in mitotic and 14 to 68 among meiocytes in the first cycle regenerants. The range in chromosome number decreased in subsequent regeneration cycles. Fragmented chromosomes were observed at low frequencies in both groups of cells. The high frequency of univalents at meiosis was attributed to possible elimination of chromosomes carrying meiotic pairing control genes.Key words: somaclonal variation, hybrids (intergeneric), tissue culture, chromosomal mixoploidy.\",\"PeriodicalId\":9589,\"journal\":{\"name\":\"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie\",\"volume\":\"18 1\",\"pages\":\"618-623\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1986-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"16\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1139/G86-090\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1139/G86-090","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Chromosome instability in somaclones of a Triticum crassum × Hordeum vulgare hybrid
Immature inflorescence culture and subsequent plant regeneration was practiced for four successive cycles using a Triticum crassum × Hordeum vulgare hybrid cultured on Kao's medium supplemented with 2,4-dichlorophenoxyacetic acid (5 mg/mL). In one line, chromosomal mixoploidy was observed among both mitotic and meiotic cells. Variation in chromosome number of 20 to 98 was observed in mitotic and 14 to 68 among meiocytes in the first cycle regenerants. The range in chromosome number decreased in subsequent regeneration cycles. Fragmented chromosomes were observed at low frequencies in both groups of cells. The high frequency of univalents at meiosis was attributed to possible elimination of chromosomes carrying meiotic pairing control genes.Key words: somaclonal variation, hybrids (intergeneric), tissue culture, chromosomal mixoploidy.