{"title":"人心脏α -肌动蛋白启动子驱动pEGFP-N1转染人胚胎生殖细胞","authors":"J. Hua, Yuai Yang, Shuzhen Zhuang, Z. Dou","doi":"10.1109/BMEI.2009.5305494","DOIUrl":null,"url":null,"abstract":"In order to purify the cardiomyocytes derived stem cells, we constructed an expression vector, α -actin-pEGFP-N1 which consists of EGFP gene as reporter markers, and driven by human cardiacα-actin promoter, then transfected it into human embryonic germ cells(hEGCs) by Lipofectamine2000. Fluorescent observation and immunocytochemistry staining demonstrated that transfected hEGCs could differentiate into cardiomyocyte like cells and expressed GFP. The results suggest that this system may provide a model for the study of cardiogenesis and differentiation.","PeriodicalId":6389,"journal":{"name":"2009 2nd International Conference on Biomedical Engineering and Informatics","volume":"37 1","pages":"1-4"},"PeriodicalIF":0.0000,"publicationDate":"2009-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transfection of Human Embryonic Germ(EG) Cells with Human Cardiac alpha-Actin Promoter Driving pEGFP-N1\",\"authors\":\"J. Hua, Yuai Yang, Shuzhen Zhuang, Z. Dou\",\"doi\":\"10.1109/BMEI.2009.5305494\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In order to purify the cardiomyocytes derived stem cells, we constructed an expression vector, α -actin-pEGFP-N1 which consists of EGFP gene as reporter markers, and driven by human cardiacα-actin promoter, then transfected it into human embryonic germ cells(hEGCs) by Lipofectamine2000. Fluorescent observation and immunocytochemistry staining demonstrated that transfected hEGCs could differentiate into cardiomyocyte like cells and expressed GFP. The results suggest that this system may provide a model for the study of cardiogenesis and differentiation.\",\"PeriodicalId\":6389,\"journal\":{\"name\":\"2009 2nd International Conference on Biomedical Engineering and Informatics\",\"volume\":\"37 1\",\"pages\":\"1-4\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-10-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2009 2nd International Conference on Biomedical Engineering and Informatics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/BMEI.2009.5305494\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2009 2nd International Conference on Biomedical Engineering and Informatics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/BMEI.2009.5305494","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Transfection of Human Embryonic Germ(EG) Cells with Human Cardiac alpha-Actin Promoter Driving pEGFP-N1
In order to purify the cardiomyocytes derived stem cells, we constructed an expression vector, α -actin-pEGFP-N1 which consists of EGFP gene as reporter markers, and driven by human cardiacα-actin promoter, then transfected it into human embryonic germ cells(hEGCs) by Lipofectamine2000. Fluorescent observation and immunocytochemistry staining demonstrated that transfected hEGCs could differentiate into cardiomyocyte like cells and expressed GFP. The results suggest that this system may provide a model for the study of cardiogenesis and differentiation.
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