利用酶法研究枇杷叶提取物的抗氧化和前胶原合成活性

J. Kang, S. Hyun, Hyo-Min Kim, Dan-Hee Yoo, Hang-Eui Cho, In-Chul Lee
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引用次数: 0

摘要

目的:研究济州岛枇杷叶酶助提取物(EJLE)的理化性质。方法:采用胃蛋白酶法制备EJLE。研究了EJLE的抗氧化和抗衰老作用,以确定其作为功能材料的实用性。结果:在总氨基酸分析中,分离到16种成分。在500 μg/mL浓度下,EJLE对DPPH和ABTS自由基的清除率分别为97.19±0.24%和96.91±0.02%。测定了EJLE对弹性蛋白酶的抑制作用。在成纤维细胞中,500 μg/mL的EJLE显示出约52%的前胶原合成活性。结论:我们的研究结果证实了EJLE诱导化妆品材料中的抗氧化剂,并增加皮肤上的前胶原合成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antioxidant and Pro-collagen Synthesis Activities of the Eriobotrya japonica Leaf Extract Using Enzyme
Purpose: We conducted a study that examined the physicochemical properties of Eriobotrya japonica leaf enzyme-assisted extract (EJLE) from Jeju Island.Methods: EJLE was prepared using the pepsin enzyme. The antioxidant and antiaging effects of EJLE were investigated to determine its utility as a functional material.Results: In the analysis of total amino acids, 16 kinds of components were isolated from EJLE. The DPPH and ABTS radical scavenging activities of EJLE were found to be 97.19±0.24% and 96.91±0.02% respectively in 500 μg/mL. Inhibition activities of elastase by EJLE were confirmed. In fibroblasts, EJLE at 500 μg/mL demonstrated a procollagen synthesis activity of approximately 52%.Conclusion: Our results confirmed that EJLE induced antioxidants in cosmetic materials and increased procollagen synthesis on the skin.
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