亚洲山区重要生态针叶树刺柏EST-SSR标记的开发与鉴定

IF 1.1 4区农林科学 Q3 FORESTRY

Silvae Genetica Pub Date : 2020-01-01 DOI:10.2478/sg-2020-0016

Tsam Ju, Perla Farhat, W. Tao, Jibin Miao, Jialiang Li, Xiaoyan Fan, Sonam Tso, Kangshan Mao

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引用次数: 2

摘要

摘要:刺柏(Juniperus squamata)是亚洲特有针叶树，具有重要的生态和经济价值。然而，由于缺乏高度多态性的分子标记，对其遗传多样性和群体结构知之甚少。本研究为刺柏(Juniperus squamata)开发了表达序列标签微卫星标记(EST-SSR)。利用Illumina HiSeq数据通过从头组装重建该物种的转录组。基于该转录组设计了18个SSR标记，并成功扩增。只有1个位点因检测到零等位基因而被淘汰，其余17个位点为多态性位点，每个位点产生5 ~ 14个等位基因。标记交叉扩增试验在两个亲缘关系较近的种间获得成功。这些标记将为进一步研究刺槐的遗传多样性和种群结构提供基础。此外，在面对全球气候变化的情况下，它们可能有助于促进该物种的可持续森林管理战略。

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Development and Characterization of EST-SSR Markers for Juniperus squamata (Cupressaceae), an ecologically important conifer in Asian mountains

Abstract Juniperus squamata, an endemic conifer of Asia, is an important shrub ecologically and economically. Yet little is known about its genetic diversity and population structure due to lacking of highly polymorphic molecular markers. In this study, expressed sequence tag microsatellite markers (EST-SSR) were developed for Juniperus squamata. Illumina HiSeq data were used to reconstruct the transcriptome of this species by de novo assembly. Based on this transcriptome, 18 SSR markers were designed and successfully amplified. Just one locus was eliminated due to its detection of null alleles and the remaining 17 loci were polymorphic, generating five to 14 alleles per locus in J. squamata. Markers cross-amplification tests were successful in two closely related species of J. squamata. These markers will serve as a basis for further studies to assess the genetic diversity and population structure of J. squamata. As well, they could be useful in promoting sustainable forest management strategies for this species in the face of global climate change.

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来源期刊

Silvae Genetica 农林科学-林学

CiteScore

2.20

自引率

10.00%

发文量

审稿时长

3 months

期刊介绍： Silvae Genetica is an international peer reviewed journal with more than 65 year tradition and experience in all fields of theoretical and applied Forest Genetics and Tree breeding. It continues "Zeitschrift für Forstgenetik und Forstpflanzenzüchtung" (Journal of Forest Genetics and Forest Tree Breeding) founded by W. LANGNER in 1951.