{"title":"黄连木(Pistacia chinensis Bunge)多态性微卫星位点的分离与鉴定","authors":"Z. Ding, Jianbo Lu","doi":"10.2478/sg-2019-0005","DOIUrl":null,"url":null,"abstract":"Abstract Pistacia chinensis Bunge (Anacardiaceae) is a native, dioecious, perennial arbor, and an important bioenergy plant. Twelve microsatellite markers were developed for P. chinensis to evaluate genetic diversity and population genetic structure when this species was subject to landscape fragmentation. Twelve polymorphic microsatellite loci were developed in P. chinensis using a microsatellite-enriched genomic library based on magnetic beads. These loci were characterized in 24 individuals from three populations located on Thousand Island Lake, Zhejiang Province, China. The number of alleles per locus varied from three to sixteen. The observed and expected heterozygosity ranged from 0.1250 to 0.8750 and 0.2333 to 0.8917, respectively. These microsatellite loci will be applied in further studies on the population genetic diversity and genetic structure of P. chinensis. This study will improve understanding of the effects of landscape fragmentation, and help conserve and manage the species.","PeriodicalId":21834,"journal":{"name":"Silvae Genetica","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Isolation and characterization of polymorphic microsatellite loci for Pistacia chinensis Bunge (Anacardiaceae)\",\"authors\":\"Z. Ding, Jianbo Lu\",\"doi\":\"10.2478/sg-2019-0005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Pistacia chinensis Bunge (Anacardiaceae) is a native, dioecious, perennial arbor, and an important bioenergy plant. Twelve microsatellite markers were developed for P. chinensis to evaluate genetic diversity and population genetic structure when this species was subject to landscape fragmentation. Twelve polymorphic microsatellite loci were developed in P. chinensis using a microsatellite-enriched genomic library based on magnetic beads. These loci were characterized in 24 individuals from three populations located on Thousand Island Lake, Zhejiang Province, China. The number of alleles per locus varied from three to sixteen. The observed and expected heterozygosity ranged from 0.1250 to 0.8750 and 0.2333 to 0.8917, respectively. These microsatellite loci will be applied in further studies on the population genetic diversity and genetic structure of P. chinensis. This study will improve understanding of the effects of landscape fragmentation, and help conserve and manage the species.\",\"PeriodicalId\":21834,\"journal\":{\"name\":\"Silvae Genetica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2019-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Silvae Genetica\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2478/sg-2019-0005\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"FORESTRY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Silvae Genetica","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2478/sg-2019-0005","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FORESTRY","Score":null,"Total":0}
Isolation and characterization of polymorphic microsatellite loci for Pistacia chinensis Bunge (Anacardiaceae)
Abstract Pistacia chinensis Bunge (Anacardiaceae) is a native, dioecious, perennial arbor, and an important bioenergy plant. Twelve microsatellite markers were developed for P. chinensis to evaluate genetic diversity and population genetic structure when this species was subject to landscape fragmentation. Twelve polymorphic microsatellite loci were developed in P. chinensis using a microsatellite-enriched genomic library based on magnetic beads. These loci were characterized in 24 individuals from three populations located on Thousand Island Lake, Zhejiang Province, China. The number of alleles per locus varied from three to sixteen. The observed and expected heterozygosity ranged from 0.1250 to 0.8750 and 0.2333 to 0.8917, respectively. These microsatellite loci will be applied in further studies on the population genetic diversity and genetic structure of P. chinensis. This study will improve understanding of the effects of landscape fragmentation, and help conserve and manage the species.
期刊介绍:
Silvae Genetica is an international peer reviewed journal with more than 65 year tradition and experience in all fields of theoretical and applied Forest Genetics and Tree breeding. It continues "Zeitschrift für Forstgenetik und Forstpflanzenzüchtung" (Journal of Forest Genetics and Forest Tree Breeding) founded by W. LANGNER in 1951.