黄连木(Pistacia chinensis Bunge)多态性微卫星位点的分离与鉴定

IF 1.1 4区 农林科学 Q3 FORESTRY
Z. Ding, Jianbo Lu
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引用次数: 1

摘要

摘要黄连木(Pistacia chinensis Bunge)是一种乡土、雌雄异株、多年生乔木植物,是一种重要的生物能源植物。利用12个微卫星标记对柽柽树在景观破碎化过程中的遗传多样性和群体遗传结构进行了评价。利用基于磁珠的微卫星富集基因组文库,在中华水杨中发现了12个多态微卫星位点。这些位点在浙江千岛湖3个种群的24个个体中均有分布。每个位点的等位基因数量从3到16不等。观察到的杂合度为0.1250 ~ 0.8750,期望杂合度为0.2333 ~ 0.8917。这些微卫星基因座将用于进一步研究中华水杨的群体遗传多样性和遗传结构。该研究将提高对景观破碎化影响的认识,并有助于物种的保护和管理。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and characterization of polymorphic microsatellite loci for Pistacia chinensis Bunge (Anacardiaceae)
Abstract Pistacia chinensis Bunge (Anacardiaceae) is a native, dioecious, perennial arbor, and an important bioenergy plant. Twelve microsatellite markers were developed for P. chinensis to evaluate genetic diversity and population genetic structure when this species was subject to landscape fragmentation. Twelve polymorphic microsatellite loci were developed in P. chinensis using a microsatellite-enriched genomic library based on magnetic beads. These loci were characterized in 24 individuals from three populations located on Thousand Island Lake, Zhejiang Province, China. The number of alleles per locus varied from three to sixteen. The observed and expected heterozygosity ranged from 0.1250 to 0.8750 and 0.2333 to 0.8917, respectively. These microsatellite loci will be applied in further studies on the population genetic diversity and genetic structure of P. chinensis. This study will improve understanding of the effects of landscape fragmentation, and help conserve and manage the species.
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来源期刊
Silvae Genetica
Silvae Genetica 农林科学-林学
CiteScore
2.20
自引率
10.00%
发文量
10
审稿时长
3 months
期刊介绍: Silvae Genetica is an international peer reviewed journal with more than 65 year tradition and experience in all fields of theoretical and applied Forest Genetics and Tree breeding. It continues "Zeitschrift für Forstgenetik und Forstpflanzenzüchtung" (Journal of Forest Genetics and Forest Tree Breeding) founded by W. LANGNER in 1951.
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